DOI: 10.1016/j.marpolbul.2017.06.044
Scopus记录号: 2-s2.0-85021193536
论文题名: Phospholipid fatty acid (PLFA) analysis for profiling microbial communities in offshore produced water
作者: Fan F. ; Zhang B. ; Morrill P.L.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2017
卷: 122, 期: 2018-01-02 起始页码: 194
结束页码: 206
语种: 英语
英文关键词: GC-MS
; Microbial communities
; Offshore produced water
; PLFA
; Solid phase extraction
Scopus关键词: Extraction
; Gas chromatography
; Mass spectrometry
; Microorganisms
; Organic solvents
; Petroleum reservoir evaluation
; Phase separation
; Phospholipids
; Produced Water
; Recovery
; Transesterification
; Fatty acid methyl esters (FAMEs)
; Gas chromatography-mass spectrometries (GC-MS)
; GC-MS
; Microbial communities
; Phospholipid fatty acid analysis
; PLFA
; Relative standard deviations
; Solid-phase extraction
; Fatty acids
; 1,2 dinonadecanoyl sn glycero 3 phosphocholine
; 1,2 dipalmitoleoyl sn glycero 3 phosphocholine
; acetone
; dioleoylphosphatidylcholine
; fatty acid
; fatty acid ester
; glycerophospholipid
; methanol
; offshore produced water
; unclassified drug
; water
; fatty acid
; water
; extraction method
; fatty acid
; gas chromatography
; mass spectrometry
; microbial community
; neutralization
; optimization
; phospholipid
; purification
; Article
; chemical phenomena
; controlled study
; derivatization
; fatty acid analysis
; limit of detection
; mass fragmentography
; microbial community
; neutralization
; phase partitioning
; purification
; quantitative analysis
; reaction time
; solid phase extraction
; solid phase extraction column
; temperature
; transesterification
; water sampling
; environmental monitoring
; microbiology
; procedures
; water pollutant
; Environmental Monitoring
; Fatty Acids
; Gas Chromatography-Mass Spectrometry
; Solid Phase Extraction
; Water
; Water Microbiology
; Water Pollutants, Chemical
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: A method based on phospholipid fatty acid (PLFA) analysis for profiling microbial communities in offshore produced water was optimized. The operation parameters affecting final PLFA profiling performance from the solid phase extraction (SPE) purification and fatty acid methyl esters (FAMEs) yielding process were investigated. Under the selected conditions, 92.9%, 96.3% and 92.8% of the spiked phospholipid standards C16:1 (cis-9) PC, C18:1 (cis-9) PC, and C19:0 PC were recovered, respectively, using 10 mL methanol as elution solvent on a non-commercial SPE column. Over 90% of spiked C19:0 PC was recovered before sample transesterification. Four parameters including alkaline reagent, volume of acid for neutralization, time and temperature for FAMEs derivatization were examined. Gas Chromatography-Mass Spectrometry (GC-MS) was used to analyze FAMEs and the method linearities, recoveries of 29 FAMEs during transesterification, detection limits, relative standard deviations were presented. The results provided valuable information for biological reservoir souring control. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/88165
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Northern Region Persistent Organic Pollution Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University of Newfoundland, St. John's, NL, Canada; Earth Sciences, Faculty of Science, Memorial University of Newfoundland, St. John's, NL, Canada
Recommended Citation:
Fan F.,Zhang B.,Morrill P.L.. Phospholipid fatty acid (PLFA) analysis for profiling microbial communities in offshore produced water[J]. Marine Pollution Bulletin,2017-01-01,122(2018-01-02)