DOI: 10.1016/j.marpolbul.2017.12.001
Scopus记录号: 2-s2.0-85037357594
论文题名: T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro
作者: Kleinert C. ; Lacaze E. ; Fortier M. ; Hammill M. ; De Guise S. ; Fournier M.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2018
卷: 127 起始页码: 225
结束页码: 234
语种: 英语
英文关键词: Immunotoxicity
; Marine mammal
; Pharmaceuticals
; Phoca vitulina
Scopus关键词: Antibiotics
; Aquatic organisms
; Biochemistry
; Blood
; Cell culture
; Cell proliferation
; Drug products
; Lymphocytes
; Mammals
; Mixtures
; Aquatic environments
; Immunotoxicity
; Lymphocyte proliferation
; Marine mammals
; Peripheral blood mononuclear cells
; Phoca vitulina
; Predictive values
; Proliferative response
; Binary mixtures
; carbamazepine
; erythromycin
; ethinylestradiol
; naproxen
; cell organelle
; concentration (composition)
; drug
; immune response
; marine mammal
; pinniped
; toxicity test
; animal cell
; Article
; B-cell lymphoma cell line
; cell isolation
; cell proliferation
; cell viability
; concentration (parameters)
; controlled study
; cytotoxicity test
; environmental exposure
; female
; gender
; IC50
; immune response
; in vitro study
; male
; nonhuman
; peripheral blood mononuclear cell
; Phoca vitulina
; predictive value
; sensitivity analysis
; T lymphocyte
; Mammalia
; Phoca
; Phoca vitulina
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: The ubiquity of pharmaceuticals in the aquatic environment and the accumulation in organisms of lower trophic levels have been documented. The immunotoxicity of these xenobiotics has however been little investigated. This study assessed the effects of pharmaceuticals on the immune responses of harbor seal lymphocytes. Peripheral blood mononuclear cells isolated from harbor seal pups were exposed to varying concentrations of 17α-ethinyl estradiol (250–50,000 μg/L), naproxen (500–100,000 μg/L), carbamazepine (500–100,000 μg/L), erythromycin (750–150,000 μg/L) and binary mixtures thereof in vitro. All individual compounds and mixtures inhibited lymphocyte proliferation. Mixture effects were non-additive and predictive values overestimated the inhibition of proliferation. Male pups were more sensitive to erythromycin exposure. Comparison with the sensitivity of the 11B7501 cell line showed a higher sensitivity of pups to individual compounds and the inverse trend for mixtures. Based on our results, we hypothesize that pharmaceuticals may have the potential to interrupt immune functions in harbor seals. © 2017 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/88514
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: INRS-Institut Armand-Frappier, Edifice 18, 531 Blvd. des Prairies, Laval, QC, Canada; Institut Maurice-Lamontagne, Fisheries and Oceans Canada, 850, route de la Mer, C. P. 1000, Mont-Joli, QC, Canada; Department of Pathobiology, University of Connecticut, 61 North Eagleville Road, U-3089, Storrs, CT, United States
Recommended Citation:
Kleinert C.,Lacaze E.,Fortier M.,et al. T lymphocyte-proliferative responses of harbor seal (Phoca vitulina) peripheral blood mononuclear cells (PBMCs) exposed to pharmaceuticals in vitro[J]. Marine Pollution Bulletin,2018-01-01,127