DOI: 10.1016/j.marpolbul.2017.11.042
Scopus记录号: 2-s2.0-85035107200
论文题名: A cross-taxa study using environmental DNA/RNA metabarcoding to measure biological impacts of offshore oil and gas drilling and production operations
作者: Laroche O. ; Wood S.A. ; Tremblay L.A. ; Ellis J.I. ; Lear G. ; Pochon X.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2018
卷: 127 起始页码: 97
结束页码: 107
语种: 英语
英文关键词: Bacteria 16S
; Benthic ecology
; Biomonitoring
; Eukaryotes 18S
; Foraminifera 18S
; High-throughput sequencing
Scopus关键词: Animals
; Bacteria
; Biodegradation
; Biodiversity
; Ecology
; Ecosystems
; Offshore drilling
; Offshore oil wells
; Oil well drilling
; Surveys
; Well drilling
; Benthic ecologies
; Biomonitoring
; Eukaryotes 18S
; Foraminifera 18S
; High-throughput sequencing
; Offshore oil well production
; DNA
; environmental DNA
; environmental RNA
; hydrocarbon
; petroleum
; RNA
; unclassified drug
; bacterium
; benthos
; biomonitoring
; DNA
; drilling
; eukaryote
; foraminifera
; gas production
; oil production
; RNA
; Alcanivorax
; Article
; bacterium
; controlled study
; DNA barcoding
; eukaryote
; foraminifer
; macrofauna
; microbial community
; Microbulbifer
; nonhuman
; offshore oil industry
; RNA barcoding
; sea pollution
; Alcanivorax
; Bacteria (microorganisms)
; Eukaryota
; Foraminifera
; Microbulbifer
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Standardized ecosystem-based monitoring surveys are critical for providing information on marine ecosystem health. Environmental DNA/RNA (eDNA/eRNA) metabarcoding may facilitate such surveys by quickly and effectively characterizing multi-trophic levels. In this study, we assessed the suitability of eDNA/eRNA metabarcoding to evaluate changes in benthic assemblages of bacteria, Foraminifera and other eukaryotes along transects at three offshore oil and gas (O&G) drilling and production sites, and compared these to morphologically characterized macro-faunal assemblages. Bacterial communities were the most responsive to O&G activities, followed by Foraminifera, and macro-fauna (the latter assessed by morphology). The molecular approach enabled detection of hydrocarbon degrading taxa such as the bacteria Alcanivorax and Microbulbifer at petroleum impacted stations. Most identified indicator taxa, notably among macro-fauna, were highly specific to site conditions. Based on our results we suggest that eDNA/eRNA metabarcoding can be used as a stand-alone method for biodiversity assessment or as a complement to morphology-based monitoring approaches. © 2017 Elsevier Ltd Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/88533
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Coastal and Freshwater Group, Cawthron Institute, Private Bag 2, Nelson, New Zealand; School of Biological Sciences, University of Auckland, Private Bag 349, Warkworth, New Zealand; Environmental Research Institute, The University of Waikato, Private Bag 3105, Hamilton, New Zealand; King Abdullah University of Science and Technology (KAUST), Red Sea Research Centre, Thuwal, Saudi Arabia; Institute of Marine Science, University of Auckland, Private Bag 349, Warkworth, New Zealand
Recommended Citation:
Laroche O.,Wood S.A.,Tremblay L.A.,et al. A cross-taxa study using environmental DNA/RNA metabarcoding to measure biological impacts of offshore oil and gas drilling and production operations[J]. Marine Pollution Bulletin,2018-01-01,127