DOI: 10.1016/j.marpolbul.2017.12.056
Scopus记录号: 2-s2.0-85040099126
论文题名: Spectroscopic analyses and genotoxicity of dioxins in the aquatic environment of Alexandria
作者: Abdel-Gawad F.K. ; Osman O. ; Bassem S.M. ; Nassar H.F. ; Temraz T.A. ; Elhaes H. ; Ibrahim M.
刊名: Marine Pollution Bulletin
ISSN: 0025-326X
EISSN: 1879-3363
出版年: 2018
卷: 127 起始页码: 618
结束页码: 625
语种: 英语
英文关键词: Dicentrarchus labrax
; Dioxins
; FTIR
; HF/3-21g ∗∗
; Mediterranean Sea
; Pagrus auratus
Scopus关键词: Biochemistry
; Fish
; Fourier transform infrared spectroscopy
; Gene expression
; Genes
; Molecular modeling
; Muscle
; Polymerase chain reaction
; Spectrometers
; Spectroscopic analysis
; Water pollution
; Dicentrarchus labrax
; Dioxins
; FTIR
; Mediterranean sea
; Pagrus auratus
; Organic pollutants
; 2,8 dichloro dibenzo para dioxin
; alanine
; dioxin
; major histocompatibility antigen class 1
; major histocompatibility antigen class 3
; transferrin
; unclassified drug
; aquatic environment
; concentration (composition)
; dioxin
; FTIR spectroscopy
; gene expression
; genotoxicity
; perciform
; animal tissue
; aquatic environment
; Article
; calculation
; concentration (parameters)
; controlled study
; gene expression regulation
; genotoxicity
; high performance liquid chromatography
; immunogenetics
; infrared spectroscopy
; molecular model
; nonhuman
; process optimization
; reverse transcription polymerase chain reaction
; spectroscopy
; structure analysis
; ultraviolet radiation
; upregulation
; water pollution
; Abu Qir Bay
; Egypt
; Mediterranean Sea
; Chrysophrys auratus
; Dicentrarchus labrax
Scopus学科分类: Agricultural and Biological Sciences: Aquatic Science
; Earth and Planetary Sciences: Oceanography
; Environmental Science: Pollution
英文摘要: Dioxins have global concerns because of the bioaccumulation tendency and persistency in the environment. Water, seabream Pagrus auratus and seabass Dicentrarchus labrax samples were collected from Abu Qir, Alexandria to evaluate the concentration of dioxin. Fourier Transform Infrared Spectrometer (FTIR) and molecular modeling was applied for elucidating the molecular structure of fish samples. Furthermore, HPLC with UV detection was used to determine the concentration of dioxins (2,8-dichloro dibenzo-p-dioxin). RT-PCR assay was conducted to verify the expression of some immune genes in the fish species as a result of water pollution. The average detected concentrations varied from 0.2 to 1.3 μg/l. Gene expression revealed that MHC class 1 and C3 were highly upregulated in liver and muscle of seabass and seabream while T2BP was highly regulated in seabass liver and seabream muscle and seabass muscle for transferrin, FTIR and molecular modeling indicate that dioxin finds its way to fish protein. © 2017 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/88546
Appears in Collections: 过去全球变化的重建 全球变化的国际研究计划
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作者单位: Environmental Research Division, Centre of Excellence for Advanced Science, National Research Centre, 33 El-Bohouth St., Dokki, Giza, Egypt; Spectroscopy Department, National Research Centre, 33 El-Bohouth St., Dokki, Giza, Egypt; Environmental Sciences and Industrial Development Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Beni-Suef, Egypt; Marine Science Department, Faculty of Science, Suez Canal University, Ismailia, Egypt; Faculty of Women for Arts, Science and Education, Physics Department, Ain Shams University, Cairo, Egypt
Recommended Citation:
Abdel-Gawad F.K.,Osman O.,Bassem S.M.,et al. Spectroscopic analyses and genotoxicity of dioxins in the aquatic environment of Alexandria[J]. Marine Pollution Bulletin,2018-01-01,127