| 项目编号: | 1706731
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| 项目名称: | Control and estimation of glycosylation profile via media supplementation based on intracellular models in mammalian cell cultures |
| 作者: | Seongkyu Yoon
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| 承担单位: | University of Massachusetts Lowell
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| 批准年: | 2017
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| 开始日期: | 2017-09-01
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| 结束日期: | 2020-08-31
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| 资助金额: | 329525
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | glycosylation
; media supplementation
; culture perturbation
; control strategy
; mab quality control
; mab glycosylation
; intracellular metabolic model
; control methodology
; successful control
; intracellular property
; glycosylation variation
; media supplement
; intracellular metabolic mechanism
; glycan profile
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| 英文摘要: | Recombinant monoclonal antibodies (mAbs) are effective in curing many diseases, including several types of cancer. The effectiveness of mAbs is closely related to the pattern of decoration of the mAb surface with sugars. This project attempts to understand and ultimately predict how mAbs are decorated with sugars in specific locations on the surface. Such knowledge would fill a significant gap in current industrial practice on mAb quality control and improve the development and manufacturing of such therapeutic molecules. The research team will also provide STEM training opportunities through undergraduate research, recruiting minority students, developing new biomanufacturing courses and lectures, and supporting outreach activities for K-12 students and general public.
Successful control and optimization of mAb glycosylation is currently hindered by limited understanding of how glycosylation responds in vivo to culture perturbations. This research proposes to tackle the challenge by exploring intracellular metabolic mechanisms that link media supplementation in to glycosylation.This should eventually lead to the establishment of a model to predict glycosylation variation in cultures and accommodate a control methodology via media supplementation. Mechanistic insights into the cellular physiology critical to glycosylation will be achieved by multi-omics approaches. This will pave the way to developing intracellular metabolic models which connect media supplements, intracellular properties, and glycan profiles, an to developing control strategies for glycan attributes via media supplementation. If successful, these results should enable the reproducible generation of fully active monoclonal antibodies, increasing the efficacy and decreasing the cost of this important human health therapy. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/89026
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Seongkyu Yoon. Control and estimation of glycosylation profile via media supplementation based on intracellular models in mammalian cell cultures. 2017-01-01.
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