| 项目编号: | 1705384
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| 项目名称: | Correlating Nanoparticle-Induced Biomembrane Perturbation with Heterogeneous Surface Chemistry |
| 作者: | Yan Yu
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| 承担单位: | Indiana University
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| 批准年: | 2017
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| 开始日期: | 2017-09-01
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| 结束日期: | 2020-08-31
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| 资助金额: | 350000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | nanoparticle
; biomembrane
; heterogeneous surface chemistry
; non-uniform surface coating
; surface coating
; surface chemistry
; function
; heterogeneous surface
; controlled surface property
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| 英文摘要: | Engineered nanoparticles (particles of inorganic matter with a diameter on the order of tens of nanometers) have made inroads into many technology and industry sectors, from food, electronics, manufacture, consumer products, to medicine. As the use of nanoparticles continues to surge, it is critical to assess how the exposure to nanoparticles impacts human health. One major challenge, among many, is to understand the potential toxicity introduced by the surface coatings on the nanoparticles. To tackle this challenge, this research project is specifically designed to reveal the effect of non-uniform surface coatings of nanoparticles on the structure and function of biomembranes. Nanoparticles are created to display non-uniform surface coatings that resemble engineered nanoparticles found in the environment. Investigations on the interactions between the nanoparticles and the biomembranes will provide guidelines to better predict the biological impact of industrial particles. This knowledge will also enable the design of nanoparticles with controlled surface properties, achieving desirable biological impacts. Educational and outreach activities implemented by the researchers will popularize sciences at the interface of nanotechnology and biology. The use of a "Nano-Bio Ambassadors" program, in particular, will bring nano-bioscience to students in rural communities in Indiana as well as to the general public.
The overall objective of this project is to establish a quantitative understanding of how nanoparticles with heterogeneous surface chemistry influence the structure and function of biological membranes. Towards that goal, the researchers propose to focus on heterogeneous surfaces comprised of hydrophobic and charged groups, because this resembles the surface chemistry of industrial nanoparticles in water. Three specific aims are pursued. First, a series of amphiphilic nanoparticles that vary over a range of sizes and in hydrophilic-lipophilic balance are synthesized. Second, the disruption of such nanoparticles to the integrity and function of supported lipid bilayers is investigated and the underlying molecular mechanisms are determined. Third, the effects of membrane properties, including curvature, composition, and lipid raft formation, on interactions between amphiphilic nanoparticles and biomembranes is elucidated using the giant lipid vesicle system. Changes in lipid membranes induced by nanoparticles is mostly quantified using advanced optical and electron microscopies. Findings from this research establishes a direct connection between heterogeneous surface chemistry in nanoparticles and their perturbation to biomembranes. This study provides knowledge that is much needed but currently non-existent: a quantitative understanding of how a heterogeneous surface chemistry impacts interactions between nanoparticles and biomembranes. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/89030
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Yan Yu. Correlating Nanoparticle-Induced Biomembrane Perturbation with Heterogeneous Surface Chemistry. 2017-01-01.
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