globalchange  > 全球变化的国际研究计划
项目编号: 1705874
项目名称:
CuO NP bioactivity in the wheat rhizosphere: Interplay of soil chemistry, root exudation, and biofilms
作者: David Britt
承担单位: Utah State University
批准年: 2017
开始日期: 2017-08-01
结束日期: 2020-07-31
资助金额: 308162
资助来源: US-NSF
项目类别: Standard Grant
国家: US
语种: 英语
特色学科分类: Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
英文关键词: soil component ; root metabolite ; nanoparticle ; biofilm formation ; interplay ; plant-root interface ; plant root zone ; complex soil solution environment ; nanoparticle bioactivity ; field-grown wheat ; soil pore-water ; response ; research project ; plant root ; pseudomonad root surface colonizer
英文摘要: This research project explores copper oxide nanoparticle activity at the plant-root interface. Copper oxide is a widely-used nanoparticle (a particle with a diameter below 100 nm) in fungicidal and fertilizer applications; however, plant responses to this material span from toxicity to plant health over a very narrow concentration range. The association of roots with beneficial microbes, both as internal, or endophytic, and external, or epiphytic, colonizers, will influence nanoparticle bioactivity in this zone. A systems approach is employed in this research to identify and elucidate key aspects of nanoparticle-microbe-plant-soil component interactions to better predict the environmental and health implications of this broadly used class of nanoparticles. Detailing the interaction and impact of nanoparticles in the plant root zone colonized with defined bacteria commonly associated with field-grown plants will improve the understanding of the important role of the plant microbiome as well as contribute to guidelines for the safe handling, management, and utilization of engineered nanoparticles. While focusing on a plant system, this research project relates to the essential roles that beneficial microbes play in the health and survival of many organisms, thus the research and associated outreach will augment the growing network of microbiome studies.

The main objective of this research project is to identify the combined roles of three major factors, the plant, colonizing microbes, and soil components, that interplay to condition the bioactivity of nanoparticles in the rhizosphere, the zone vital to plant health that exists around a plant root. Two model systems that mimic key elements governing this interplay are being employed. The first system uses a solid growth matrix of silica sand amended with characterized soil pore-waters in order to replicate a key feature of the complex soil solution environment, including inorganic and organic components. Wheat grown in this system will be colonized by microbial isolates from field-grown wheat: A bacillus endophyte isolated from seeds, and a pseudomonad root surface colonizer. These microbes are typical of many isolates that induce plant tolerance to abiotic and biotic stress, such as drought and pathogens. The second system is a root-mimetic, hollow fiber membrane on which microbe colonization, metabolite production, and biofilm formation are being investigated in response to defined ?rhizosolutions? delivered through the fiber lumen. Rhizosolutions constructed from characterized root metabolites permit identification of root metabolites and soil components influencing biofilm formation, bacterial resilience to nanoparticle exposure, and metabolite production in response to the copper oxide nanoparticles, contrasted with responses from bulk copper oxide as well as copper salts. This interdisciplinary project, which merges engineering with chemical and biological sciences, is supporting graduate, undergraduate, and high school students, and underrepresented students from the Utah State University regional campuses through the Native American Summer Mentorship Program (NASMP) in place in the investigators? laboratories. STEM outreach to the general public and K-12 students is involving collaboration with an ARTsySTEM initiative, designed to better link art with science.
资源类型: 项目
标识符: http://119.78.100.158/handle/2HF3EXSE/89560
Appears in Collections:全球变化的国际研究计划
科学计划与规划

Files in This Item:

There are no files associated with this item.


Recommended Citation:
David Britt. CuO NP bioactivity in the wheat rhizosphere: Interplay of soil chemistry, root exudation, and biofilms. 2017-01-01.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[David Britt]'s Articles
百度学术
Similar articles in Baidu Scholar
[David Britt]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[David Britt]‘s Articles
Related Copyright Policies
Null
收藏/分享
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.