| 项目编号: | 1704901
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| 项目名称: | Tuning Interfacial Biomolecule Interactions with Massively Parallel Nanopore Arrays |
| 作者: | Matthew Kipper
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| 承担单位: | Colorado State University
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| 批准年: | 2017
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| 开始日期: | 2017-07-15
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| 结束日期: | 2020-06-30
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| 资助金额: | 410894
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | dna
; interaction
; protein crystal
; couple dna-pore interaction
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| 英文摘要: | DNA stores biological information with a very high density. New tools of genomics and genetic engineering that have emerged over the past 20 years, providing the transformative capabilities to both "read" DNA, and to "write" DNA. New biosensors that diagnose diseases and predict responses to treatments can be based on "reading" DNA, and development of new pest-resistant crops, for example, can be based on "writing" DNA. While DNA is protected when inside living cells, it is relatively unstable when exposed to environments outside of cells. This work will advance our understanding of how DNA interacts with a new class of materials with DNA-sized, nanoscale pores. The chemistry of these pores can be tuned to optimize interactions with DNA, for storage, stability, and for reporting specific chemical binding events. In future work, these materials could be used to develop new biosensors and advanced nanomaterials that could store DNA or transduce mechanical and chemical signals through controlled DNA binding.
We will study the interactions of short DNA segments with the surfaces and pores of crosslinked protein crystals, by atomic force microscopy and adsorption isotherm measurements. Diffusion of DNA within nanopores will be characterized by fluorescence microscopy methods. Finally, we will assess the ability of protein crystals to: stabilize guest DNA, couple DNA-pore interactions to ligand binding on the protein crystal surface, and report DNA hybridization through fluorescence. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/89761
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Matthew Kipper. Tuning Interfacial Biomolecule Interactions with Massively Parallel Nanopore Arrays. 2017-01-01.
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