| 项目编号: | 1707194
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| 项目名称: | Automated target concentration, signal enhancement, and detection in a single point-of-care paper-based diagnostic |
| 作者: | Daniel Kamei
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| 承担单位: | University of California-Los Angeles
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| 批准年: | 2017
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| 开始日期: | 2017-07-01
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| 结束日期: | 2020-06-30
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| 资助金额: | 300000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | pre-concentration
; signal enhancement reagent
; low detection limit
; detection limit
; signal enhancement capability
; lfa detection zone
; target biomarker
; signal enhancement reaction
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| 英文摘要: | The goal of this project is to develop an easy to use sensor device (similar to the over-the counter pregnancy test) for the detection of malarial infection at a sensitivity suitable for diagnosis. The sensor device is expected to be robust, rapid, low-cost, easy-to-interpret and have a low detection limit while requiring no equipment or trained personnel. The primary goal is to significantly improve the detection limit while maintaining all the desirable operating characteristics.
Available diagnostic methods for malaria have excellent sensitivities and specificities, but are not suitable for resource-poor settings. Current lateral-flow immunoassay (LFA) devices detect Plasmodium lactate dehydrogenase (pLDH), Plasmodium-specific histidine-rich protein (HRP-2), and/or Plasmodium aldolase, all of which are found in an infected individual, but their sensitives are below the recommended World Health Organization threshold of 95% sensitivity. The goal of the proposed work is to improve the sensitivity of the conventional LFA, by integrating two methods of enhancement into a single, automated paper-based device. The first method utilizes aqueous two-phase system (ATPS) separation on paper, in which a well-mixed ATPS solution rapidly separates into its macroscopic phases and concentrates the target biomarker as it flows through a paper membrane. The second method incorporates a signal enhancement reaction that utilizes engineered nanoparticles with enzymatic activity. The proposed work will investigate a new approach for controlling fluid flow through the extension of ATPS separation on paper in order to sequentially deliver signal enhancement reagents to the LFA detection zone. Additionally, to maximize shelf-life and ease-of-use, all required assay components will be dehydrated on the LFA test strip. It is anticipated that the seamless coupling of the LFA with pre-concentration and signal enhancement capabilities will result in significant sensitivity when compared to the traditional LFA, while maintaining the same user-friendliness. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/89775
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Daniel Kamei. Automated target concentration, signal enhancement, and detection in a single point-of-care paper-based diagnostic. 2017-01-01.
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