| 项目编号: | 1705560
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| 项目名称: | Enhancing Transgene Expression and Retention by Co-delivery of DNA Vectors with Modified Histones |
| 作者: | Chongli Yuan
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| 承担单位: | Purdue University
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| 批准年: | 2017
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| 开始日期: | 2017-07-01
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| 结束日期: | 2020-06-30
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| 资助金额: | 449998
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | expression
; transgenic dna
; gene delivery
; heterologous gene expression
; transgene expression
; eukaryotic gene expression system
; active gene expression
; protein expression
; conventional gene delivery approach
; histone protein
; established delivery approach
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| 英文摘要: | Gene therapy has been offered as a potential cure for a wide range of diseases, including hemophilia, cystic fibrosis, Parkinson's, HIV, hepatitis, and heart disease. Conventional gene delivery approaches currently lack the safety, efficiency and durability required for many research, industrial and/or clinical applications. Understanding how to create and maintain active gene expression will have broad-ranging impacts on gene delivery and expression in humans. The proposed work will bring effective gene therapies closer to reality, and will also train and inspire the next generation of scientists and engineers through their active participation in this interdisciplinary research.
The long-term objective of this project is to elucidate how long-term cellular memories of transcriptional states are created and maintained to advance research, industrial and gene therapy applications requiring regulated transgene expression. The specific scientific objectives are to 1) develop a robust strategy for co-delivering transgenes together with modified histone proteins promoting active transcription; and 2) evaluate and enhance protein expression from chromatin assembled on transgenic DNA. The established delivery approach is expected to significantly improve the uptake and controlled expression of transgenic DNA and to be compatible with a broad range of vectors. The fundamental kowledge gained regarding the design of epigenetic systems for heterologous gene expression could have profound implications for all eukaryotic gene expression systems. Such insight could, for example, significantly expand the scope and efficacy of human gene therapy, leading to cures for many serious diseases. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/89920
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Chongli Yuan. Enhancing Transgene Expression and Retention by Co-delivery of DNA Vectors with Modified Histones. 2017-01-01.
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