| 项目编号: | 1740961
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| 项目名称: | EAGER: Bio-Inspired Electrochemical Sensing of Small Molecules using Antibodies |
| 作者: | Edgar Goluch
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| 承担单位: | Northeastern University
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| 批准年: | 2017
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| 开始日期: | 2017-04-15
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| 结束日期: | 2018-01-31
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| 资助金额: | 75000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | antibody
; redox-active molecule
; small bioanalyte
; harmful molecule
; small biological molecule
; voltammetry-based electrochemical sensor
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| 英文摘要: | PI: Goluch, Edgar D.
Proposal Number: 1740961
A new approach for measuring small biological molecules such as toxins found in surface water is proposed. The approach, if successful, can provide a basis for compact devices that are portable for measuring harmful molecules.
There is a scarcity of sensors for detection of small biological molecules. The current approach - enzyme-linked immunosorbent assay (ELISA) - generally cannot be used for sensing small biomolecules due to limited binding sites on the antigen. An alternative approach is needed. Antibodies that have antigens bound to them are recognized in mammalian systems by C1q protein. It is generally accepted that all antibodies undergo a conformational change when they bind to an antigen. The change is recognized by the protein, C1q, which attaches to the conserved, Fc, region of the antibody. In the proposed work, C1q recognition event will be used to examine feasibility of detecting small bioanalytes in aqueous samples. The binding measurements and characterization will be performed using commercially available instrument systems. In the second aim, C1q protein will be modified with a redox-active molecule for measuring using voltammetry-based electrochemical sensor, as it is a simple, robust, and mature technology. If the project is successful, it is expected to be broadly applicable to many sensing application with the potential to develop a low-cost, portable electrochemical sensing platform. The project will provide undergraduate and graduate students with multidisciplinary research experiences that will foster collaboration between engineers and biologists, preparing them for future workplace environments. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/90325
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Edgar Goluch. EAGER: Bio-Inspired Electrochemical Sensing of Small Molecules using Antibodies. 2017-01-01.
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