| 项目编号: | 1653611
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| 项目名称: | CAREER: An ImmunoBioEngineering Platform for Rapid and Scalable Biomanufacturing of Universal Viral Vaccines |
| 作者: | Fei Wen
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| 承担单位: | University of Michigan Ann Arbor
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| 批准年: | 2017
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| 开始日期: | 2017-02-01
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| 结束日期: | 2022-01-31
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| 资助金额: | 500000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | universal protection
; universal flu vaccine
; current influenza vaccine
; next generation viral vaccine
; career proposal
; scalable influenza vaccine biomanufacturing platform
; yeast-based
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| 英文摘要: | PI: Wen , Fei
Proposal No: 1653611
Influenza epidemics result in 250,000-300,000 deaths and 3-5 million cases of severe illness annually. Unfortunately, current influenza vaccines offer limited protection requiring seasonal flu shots and pose pandemic susceptibility due to technological limitations of the egg-based manufacturing. Therefore, it is of great societal impact to develop a rapid and scalable system for biomanufacturing of universal flu vaccines. Towards this goal, this CAREER proposal aims to develop a yeast-based system for synthesizing and engineering artificial influenza-like viruses (AILVs) capable of inducing universal protection against influenza viruses. It represents a novel approach with the potential of shifting from current approach of simple natural virus-like particle synthesis to complex designer virus-like particle engineering for developing the next generation viral vaccines. Research findings will be used to strengthen the undergraduate and graduate curricula, which will also be used in K-12 outreach endeavors and increase public scientific literacy in the STEM fields.
Yeast-based AILVs have not been previously developed due to several molecular and cellular challenges. This proposal will address these issues using a multi-disciplinary approach interfacing protein engineering, immunology, synthetic biology, biochemistry, and biostatistics. Specifically, we will investigate: (1) if yeast is capable of producing and assembling key influenza viral proteins into functional AILVs; (2) if the resulting AILVs can elicit protective antibody and T-cell responses; and (3) the molecular design of AILVs capable of universal protection. The resulting yeast-based AILVs will not only serve as a rapid and scalable influenza vaccine biomanufacturing platform, but also will be used to probe immune responses to answer central questions in T-cell immunology, 3D self-assembly of proteins, and more. Research findings will be used to strengthen the undergraduate and graduate curricula, which will also be used in K-12 outreach endeavors and increase public scientific literacy in the STEM fields. PI plans to develop creative pedagogies that are adaptable by educators at all levels for improved student learning. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/90553
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Fei Wen. CAREER: An ImmunoBioEngineering Platform for Rapid and Scalable Biomanufacturing of Universal Viral Vaccines. 2017-01-01.
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