| 项目编号: | 1604087
|
| 项目名称: | GOALI: Biocatalysis Development using High-throughput Droplet Microfluidics and Mass Spectrometry |
| 作者: | Robert Kennedy
|
| 承担单位: | University of Michigan Ann Arbor
|
| 批准年: | 2016
|
| 开始日期: | 2016-09-01
|
| 结束日期: | 2019-08-31
|
| 资助金额: | 425000
|
| 资助来源: | US-NSF
|
| 项目类别: | Standard Grant
|
| 国家: | US
|
| 语种: | 英语
|
| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
|
| 英文关键词: | development
; reaction
; droplet microfluidic
; goali program
; pharmaceutical development project
; fast development
; mass spectrometry
; drug development project
; chemical process development
|
| 英文摘要: | 1604087
Kennedy, Robert T.
The goal of this project is to develop a new approach to facilitate the development of faster, greener, and cheaper ways of synthesizing drugs. The challenge in using enzymes is that natural occurring enzymes do not often catalyze the exact reaction that is needed for a pharmaceutical development project. The goal in this project is to develop a high-throughput, highly miniaturized method of engineering enzymes to perform desired reactions. It is anticipated that this technology can be used to develop such biocatalysts in a few days for any desired chemical transformation. This capability could greatly facilitate drug development projects by making available tailor-made catalysts for any reaction in a time and cost-effective manner.
Enzyme engineering for pharmaceutical process chemistry and manufacturing has become a revolutionary technology as enzymatic transformations potentially offer high yield, selectivity and enantiomeric purity with low cost and green chemistry. The team proposes development of a novel screening system to reduce the material use, eliminate special reagents and improve the overall throughput in enzyme engineering and screening. The three key modules include (i) cell-free protein synthesis (CFPS), (ii) droplet microfluidics and (iii) mass spectrometry (MS). CFPS offers the potential speed advantage as it bypasses the cell growth and protein harvest steps. It can be completed with shorter cycle time, produces purer proteins, and at the same time allows active monitoring, direct manipulation, and rapid sampling. Droplet microfluidics offers a platform for performing reactions and assays in nanoliter or less volume which is also adaptive to be automated. MS allows the possibility for fast development of label-free assays for a wide variety of potential substrates with minimal delay. The proposed approach is transformative because it will allow virtually any enzymatic transformation to be engineered in 10-fold less time by eliminating the need to develop labels, coupled reactions, or using cells in the process. The proposed technology will be useful also for other applications in chemical process development.
This award by the Biotechnology and Biochemical Engineering Program of the CBET Division is co-funded by the GOALI Program of the Division of Industrial Innovation and Partnerships. |
| 资源类型: | 项目
|
| 标识符: | http://119.78.100.158/handle/2HF3EXSE/91254
|
| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
|
|
There are no files associated with this item.
|
| Recommended Citation: | |
Robert Kennedy. GOALI: Biocatalysis Development using High-throughput Droplet Microfluidics and Mass Spectrometry. 2016-01-01.
|
|
|