| 项目编号: | 1604893
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| 项目名称: | INTRACELLULAR ELECTROCHEMISTRY WITH CARBON NANOTUBE-BASED SENSORS |
| 作者: | Michael Schrlau
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| 承担单位: | Rochester Institute of Tech
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| 批准年: | 2016
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| 开始日期: | 2016-09-01
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| 结束日期: | 2019-08-31
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| 资助金额: | 294103
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | single living cell
; tiny biosensor
; project
; carbon nanotube
; intracellular electrochemistry
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| 英文摘要: | PI: Schrlau, Michael
Proposal Number: 1604893
This project proposes to create a very tiny biosensor for identifying and quantifying biomolecules inside a single living cell. The investigator proposes a novel way of performing selective measurement inside living cells, (instead of currently used methods of breaking open the cell), and after proving the approach plan is to quantify cellular metabolic state in real time.
The goal of the project is to create the ability to identify and quantify biomolecules inside a single living cell with minimal perturbation and over long periods of time using electrochemical techniques. Multifunctional nanoprobe will be constructed, capable of injecting fluids in order to conduct self-contained electrochemical measurements inside confined aqueous microenvironments. The nanoprobe is then utilized to inject a reaction-enabling substrate into the cell for selectively quantifying senescence-associated beta-galactosidase, a cytosolic enzyme, an indicator of many chronic diseases. It is proposed to use carbon nanotube (CNT) -based nanoprobes will provide the ability for selectively quantifying cell senescence in real-time. It is anticipated that at the conclusion of the project, there will be a new analytical tool and technique for quantifying electroactive biomolecules within single living cells. Simple, scalable template-based nanomanufacturing processes will be employed to integrate the nanoelectrodes into the tip of a pulled glass capillary without nanoassembly. The CNT-based nanoprobe is designed to readily fit standard cell physiology instruments, thus facilitating easy technological dissemination, broad utilization, and potential commercialization. The outcome of this proposal is likely to provide a first-in-class CNT-based tool for a novel minimally invasive analytical technique for gathering quantitative data from single living cells. The proposed efforts will generate new insights into fundamental cell physiology, which will ultimately help identify the early onset of diseases, facilitate drug development and improve therapeutic efficacy. More broadly, the development of intracellular electrochemistry will advance scientific knowledge in nanotechnology, materials science and engineering, electrochemistry, cell biology, and biomedicine. Further, the project will provide several high-impact research and educational opportunities for students at all levels as well as recruits and will support underrepresented groups in STEM. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/91280
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Michael Schrlau. INTRACELLULAR ELECTROCHEMISTRY WITH CARBON NANOTUBE-BASED SENSORS. 2016-01-01.
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