| 项目编号: | 1605425
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| 项目名称: | SusCHEM: Fundamental Studies on Lyopreservation of Adult Stem Cells |
| 作者: | Xiaoming He
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| 承担单位: | Ohio State University
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| 批准年: | 2016
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| 开始日期: | 2016-09-01
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| 结束日期: | 2019-08-31
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| 资助金额: | 300000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | ambient temperature
; cell
; cell-based
; living cell
; stem cell property
; lyopreservation
; cell lyopreservation
; trehalose-laden
; cell dehydration
; cell survival
; cell-based medical treatment
; stem cell therapy
; trehalose
; trehalose-laden human adipose-derived stem cell
; stem cell gene
; systematic investigation
; cell transplantation
; primary hadsc
; cell-based therapy
; nsf suschem initiative
; cell banking
; trehalose-laden stem cell
|
| 英文摘要: | 1605425
He, Xiaoming
Lyopreservation of living cells for banking in a state of suspended animation at ambient temperature makes it possible to achieve low-cost maintenance and convenient distribution of the cells. The ready availability of cells is crucial for the wide application and eventual success of cell-based therapies involving tissue engineering, regenerative medicine, cell transplantation, blood transfusion, and stem cell therapy. The objective of this project is to perform a systematic investigation to understand the protective effect of trehalose, a nontoxic sugar, during freezing and drying of trehalose-laden human adipose-derived stem cells (hADSCs) and in banking the dried cells at ambient temperature. As trehalose has been used for many lower organisms to survive extreme cold and drought in nature, it is hypothesized that trehalose-laden stem cells will behave similarly.
This project will have a significant impact on improving healthcare by facilitating the development of cell lyopreservation as an enabling technology for the success of cell-based medical treatments. The use of a nontoxic sugar (trehalose) to replace toxic chemicals (e.g., dimethyl sulfoxide or DMSO) for cell banking is well aligned with the NSF SusCHEM initiative to replace toxic chemicals with earth-abundant, inexpensive, and benign materials. To understand the fundamental science of drying living cells for banking in a state of suspended animation at ambient temperature, three specific research tasks are proposed. These include (i) investigation of the effect of freeze-drying parameters on the kinetics of drying trehalose solutions important for lyopreservation of primary hADSCs and on the microscopic morphology of the dried products, using freeze-drying microscopy and a benchtop freeze-dryer for small and large samples, respectively; (ii) simulation and experimental quantification of the biophysical responses of the trehalose-laden hADSCs to freezing and drying including cell dehydration and intracellular ice formation important for cell survival; and (iii) analysis of the morphology, survival, long-term proliferation, and stem cell properties and functions of the primary hADSCs after freeze-drying and banking at ambient temperature. The stem cell properties and functions will be assessed by the expression of stem cell gene and protein markers and their capability of adipogenic, osteogenic, and chondrogenic differentiation. A high survival and intact function of the primary hADSCs are anticipated after freeze-drying and long-term storage at ambient temperature under optimal conditions.
This award by the Biotechnology and Biochemical Engineering Program of the CBET Division is cosponsored by the Biomaterials Program of the Division of Materials Research. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/91354
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Xiaoming He. SusCHEM: Fundamental Studies on Lyopreservation of Adult Stem Cells. 2016-01-01.
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