| 项目编号: | 1644328
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| 项目名称: | EAGER: Rapid isolation of live microbial species from environmental communities |
| 作者: | Gillian Gile
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| 承担单位: | Arizona State University
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| 批准年: | 2016
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| 开始日期: | 2016-08-01
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| 结束日期: | 2018-07-31
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| 资助金额: | 149173
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Biological Sciences - Environmental Biology
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| 英文关键词: | project
; species
; microbe
; key protozoan species
; hindgut community
; individual species
; termite species
; understudied microbial community
; zootermopsis species
; many protozoan species
; complex community
; diversity
; separate complex microbial community
; constituent species
; microbial species
; ecology community
; separate microbial community
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| 英文摘要: | Microbes are everywhere, and they play major roles in ecosystem function and human health. However, studying microbes is challenging because they are so small and because they live in complex communities. Most microbial species cannot be cultivated in the lab. Therefore, to understand the diversity, evolution, and ecology of microbes, scientists typically extract bulk DNA from the environment. This can reveal which types of microbes are present, but it does not provide information about what each species looks like and which species are carrying out which processes. In this project, researchers will develop a device to separate complex microbial communities into their individual species. The researchers will share this separation technology broadly with other scientists in the microbial systematics, diversity, and ecology communities to help accelerate research in these areas. An interactive outreach module will be developed and brought to K-12 students to help spark appreciation for science, technology, and engineering research.
This project will develop a method using dielectrophoresis to separate microbial communities based on morphotypes. Dielectrophoresis is the movement of uncharged particles in an electric field. Particles of similar size, shape, and surface characteristics move in similar ways and are expected to accumulate in the same compartment of a gated microscopic channel once an electrical field is applied. This approach will advance the fields of microbial systematics and biodiversity science by enabling scientists to link morphological and molecular biodiversity in understudied microbial communities. The separation device will be used to answer two main questions about the evolution of symbiotic, wood-digesting protozoa that live in termite hindguts. First, how did the ancestor of termite hindgut protozoa acquire the ability to digest wood? For this project, tens of thousands of cells from key protozoan species will be separated from the rest of their communities for transcriptome sequencing. Genes for wood digesting enzymes will be identified and phylogenetically analyzed along with related genes from other organisms. Second, have protozoan symbionts transferred from one termite species to another in the genus Zootermopsis? And if so, can this explain the greater diversity of protozoa relative to their hosts? For this project, the hindgut communities of four Zootermopsis species will be separated into their constituent species in order to determine how many protozoan species are present and how closely they are related to one another. This information will be compared to the phylogeny and biogeography of Zootermopsis termites to determine whether and when such transfers may have occurred. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/91624
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Gillian Gile. EAGER: Rapid isolation of live microbial species from environmental communities. 2016-01-01.
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