| 项目编号: | 1724640
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| 项目名称: | EAGER: Biomanufacturing: Cell Differentiation Bioreactor For Cardiac Tissue Engineering |
| 作者: | Jeffrey Jacot
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| 承担单位: | University of Colorado at Denver
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| 批准年: | 2016
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| 开始日期: | 2016-07-01
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| 结束日期: | 2018-08-31
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| 资助金额: | 167583
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | cardiac tissue
; tissue
; heart tissue
; pluripotent stem cell
; release
; heart defect
; closed bioreactor system
; cardiac differentiation factor
; closed-system bioreactor
; own cell
; stem cell
; tumorigenic cell
; tumorigenic undifferentiated cell
; pluripotent cell
; native tissue
; device
; differentiation factor
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| 英文摘要: | PI: Jacot, Jeffrey
Proposal Number: 1547838
Heart failure is the major cause of death in the U.S., and heart defects are the major cause of death in infants. This project will design, build and validate a device that can be used at a hospital to create living heart tissue for repair of failing hearts and heart defects. Clinicians would inject a patient's own cells into the device, which would then automatically control the release of chemicals required to make living heart muscle. In about 2 weeks, surgeons would open the device, remove the heart muscle, and use it to restructure a failing or malformed heart. This technology could transform the clinical approach to heart disease and heart defects, and could produce the first regenerative cure for these conditions.
The implementation of novel and transformative therapies using cardiac tissue derived from induced pluripotent stem cells (iPSC) requires the development of new technologies that can efficiently create heart tissue from pluripotent stem cells and ensure elimination of tumorigenic undifferentiated cells without laboratory intervention or extensive processing. This can be accomplished with a closed bioreactor system to create engineered cardiac tissue from iPSC. The intellectual merit of this proposal is based on the hypothesis that a bioreactor containing a pump, heart matrix-based hydrogel, and nanoparticles controlling the timed release of differentiation factors can create a 3-D contractile cardiac tissue with organization and function similar to native tissue. To test this hypothesis, a closed-system bioreactor that pumps media through cardiac matrix hydrogels along with silica nanoparticles that release cardiac differentiation factors will be constructed and tested with human iPSC. The release of factors shown to eliminate pluripotent cells will purify the tissue and prevent the implantation of tumorigenic cells and this will be verified through PCR and immunostaining. Finally, tissues with suturable scaffolds will be generated and implanted into a rat model of cardiac defect repair. The broader educational and social impacts of this proposal are integrated with the intellectual merit and include outreach activities with several Houston high schools though the Rice K-12 Initiative to bring increased awareness of biotechnology, biomaterials and stem cells among high school students and increased participation of high school students in science & engineering. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/91942
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Jeffrey Jacot. EAGER: Biomanufacturing: Cell Differentiation Bioreactor For Cardiac Tissue Engineering. 2016-01-01.
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