| 项目编号: | 1604562
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| 项目名称: | Visualization of mechanical stress in live cells |
| 作者: | Adam Wax
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| 承担单位: | Duke University
|
| 批准年: | 2016
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| 开始日期: | 2016-07-01
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| 结束日期: | 2019-06-30
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| 资助金额: | 599997
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | mechanical stress
; cell
; cell cortex
; live cell
; cell stress
; subcellular level
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| 英文摘要: | ABSTRACT
Wax, Adam
1604562
This proposal will develop novel optical methods for visualizing mechanical stresses in live cells. They will study the mechanics at the subcellular level to understand transduction of mechanical stresses and the roles of key proteins in both focal adhesion and the cell cortex.
The key technical advance this project will pursue is to incorporate FRET (Forster resonance energy transfer)-based tension sensors into the current quantitative phase imaging platform. Tension sensors were developed by incorporating two fluorophores into load-bearing proteins. Traditionally dynamic loading has required indirect measures and complex devices. The proposed platform will be able to directly visualize cell stresses and to do so without mechanically displacing the cell. Further technical advances will develop FRET sensors for a load-bearing protein in the cell cortex to enable simple, reproducible loading of cells while preserving imaging capabilities. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/92024
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Adam Wax. Visualization of mechanical stress in live cells. 2016-01-01.
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