| 项目编号: | 1605904
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| 项目名称: | Coupling liquid crystal phase transitions with aptamer selectivity for biomolecular sensing |
| 作者: | Raymond Tu
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| 承担单位: | CUNY City College
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| 批准年: | 2016
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| 开始日期: | 2016-06-01
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| 结束日期: | 2019-05-31
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| 资助金额: | 296898
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | liquid crystal
; crystal-aqueous
; aptamer
; aptamer selectivity
; aptamer-based analyte binding
; work
; unbound aptamer
; planar phase change
; evolvable selectivity
; cooperative phase transition
; aptamer interaction
; aptamer sequence space
; phase transition
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| 英文摘要: | PI: Tu, Raymond
Proposal Number: 1605904
The goal is to create an inexpensive and flexible biosensor using biological materials of special properties for the rapid recognition of harmful toxins and cancer biomarkers. Liquid crystals, the same structures that are in TVs and watches, can be used as an ultra-sensitive switch for visualizing the sensing phenomena. Taken together, a new device is expected to be created for the rapid detection of numerous disease biomarkers.
This work will investigate a low-cost label-free sensing technology that focuses on analytes that can quickly diagnose illnesses and toxins prevalent in impoverished populations. The researchers aim to accomplish this by combining the cooperative phase transitions of liquid crystals with the flexibility of aptamer-based analyte binding. Liquid crystal based biosensors are inexpensive and sensitive, but the label-free application of the homeotropic to planar phase change requires a change in the interfacial packing of the nematic mesogen, restricting applications to a narrow range of targets. In contrast, aptamers possess the ability to selectively bind to a wide range of analytes, and the ability to quickly evolve new targets using Systematic Evolution of Ligands by EXponential (SELEX) enrichment has made the nucleic acid recognition elements a popular topic in the sensing community. Still, the challenge remains to translate this easily evolvable selectivity into an inexpensive label-free "aptasensor." The goal of this work is to examine the fundamental interfacial science and aptamer sequence space needed to robustly couple the nematic liquid crystal-aqueous interface with aptamer selectivity and sensitivity. Our approach for creating a "generalizable" aptamer-liquid crystal system investigates in the proposed work, four fundamental aspects of the sensing system. (1) Develop a quantitative methodology for the evaluation of competitive adsorption to the nematic liquid crystal-aqueous interface. (2) Measure and model the change in surface energy as the liquid responds to surfactants, unbound aptamers and bound aptamers. (3) Determine the rate limiting steps for realistic sensor design, where the kinetics of the phase transition should not require protracted diffusive or adsorption times. (4) Develop tools for the evolution of both the aptamer selectivity as well as the aptamer interaction with the liquid crystal and surfactants. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/92178
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Raymond Tu. Coupling liquid crystal phase transitions with aptamer selectivity for biomolecular sensing. 2016-01-01.
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