| 项目编号: | 1600724
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| 项目名称: | DISSERTATION RESEARCH: Mechanisms of host preference in the ectomycorrhizal symbiosis |
| 作者: | Kabir Peay
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| 承担单位: | Stanford University
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| 批准年: | 2016
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| 开始日期: | 2016-06-01
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| 结束日期: | 2018-05-31
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| 资助金额: | 20538
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Biological Sciences - Environmental Biology
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| 英文关键词: | related host plant
; generalist host preference
; fungus
; plant
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| 英文摘要: | Although mutually beneficial interactions are important in many natural systems, it remains unclear why some involve highly specialized partners, while others do not. This project investigates how different degrees of specificity have evolved and function in a particular type of symbiosis - a mutualistic relationship between a fungus and many temperate forest plants. This research will combine a fungus with multiple, distantly related host plants to investigate how the fungus alters its symbiotic behavior when interacting with different species of plants. This experiment will generate insight into symbiotic plant-microbe communication in this important mutualism, and will allow for comparison to similar systems in pathogens. Outreach to science enrichment programs for urban youth in San Francisco will also occur.
This study will use RNA sequencing to determine the genetic basis of generalist host preference in the ectomycorrhizal fungus Thelephora terrestris. Six plant species from four families will be established in microcosms and inoculated with spores from Thelephora terrestris. At symbiotic maturity, microcosms will be labeled with carbon-13 and nitrogen-15 and plant, fungal, and symbiotic tissues will be harvested for isotope analysis and RNA sequencing. Transcriptional data will be combined with stable isotope enrichment measurements of plant and fungal resource allocation and compared across different plant-fungus combinations. Measuring gene expression in symbiotic tissues while simultaneously tracking resource allocation will reveal which genes are associated with improved symbiotic performance, providing a powerful link between gene transcription, physiological function, and the stability of mutualistic interactions. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/92203
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Kabir Peay. DISSERTATION RESEARCH: Mechanisms of host preference in the ectomycorrhizal symbiosis. 2016-01-01.
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