| 项目编号: | 1601372
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| 项目名称: | DISSERTATION RESEARCH: Metatranscriptomics links phenology with community dynamics and ecosystem function in wood decay fungi |
| 作者: | Amy Zanne
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| 承担单位: | George Washington University
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| 批准年: | 2016
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| 开始日期: | 2016-06-01
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| 结束日期: | 2018-05-31
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| 资助金额: | 19811
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Biological Sciences - Environmental Biology
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| 英文关键词: | fungus
; decay activity
; fungal community change
; specific wood component
; role wood decay fungus
; woody debris
; wood decay fungus
; wood decay fungal community
; decay gene
; community diversity
; carbon
; active fungal community
; decay gene expression
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| 英文摘要: | Forests contain half of the world's terrestrial carbon, much of which is stored over long time periods in forest trees. This carbon is typically released after tree death through the actions of fungi and other microorganisms that consume woody debris, breaking it down and releasing carbon into the soil and atmosphere (as carbon dioxide). Most of what is known about wood decay fungi comes from observations of reproductive fruiting bodies, such as mushrooms, but this is only one stage of the life cycle of these fungi. In this project, fungal activities will be studied over the course of the forest growing season and over the developmental life cycle of fungi to measure the amount of wood decay activity, and to determine the factors that control the process. These results will significantly improve our understanding of the role wood decay fungi play in releasing stored carbon now and under changing global conditions.
This project will provide needed insight into the functioning of wood decay fungal communities by examining the phenology of extracellular enzyme production throughout the year. The researchers will use recent advances in sequencing methods to determine when fungi are actively expressing decay genes, and how decay activity is affected by community diversity, substrate quality, and environmental conditions. High throughput sequencing will be used to compare decay gene expression through time by capturing populations of messenger RNAs, and to identify shifts in the active fungal community using ribosomal RNA. Sequencing data will be complemented with extracellular enzyme assays and measurements of respiration, to quantify the activity levels of enzymes targeting specific wood components and rate of carbon dioxide released to the atmosphere, respectively. The drivers of decay activity and fungal community changes will be compared with those affecting fruiting body production. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/92205
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Amy Zanne. DISSERTATION RESEARCH: Metatranscriptomics links phenology with community dynamics and ecosystem function in wood decay fungi. 2016-01-01.
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