| 项目编号: | 1554078
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| 项目名称: | CAREER: The Role of Surface Anisotropy in the Cellular Entry of Janus Particles |
| 作者: | Yan Yu
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| 承担单位: | Indiana University
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| 批准年: | 2016
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| 开始日期: | 2016-02-15
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| 结束日期: | 2021-01-31
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| 资助金额: | 500000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | janus particle
; surface anisotropy
; cellular entry
; entry
; career project
; different surface chemistry
; janus microparticle
; project
; janus sphere
; cell
; particle-cell interaction
; synthetic particle
; ligand-guided cell entry
; new single-particle tracking method
; way multifunctional drug delivery particle
; particle-level anisotropy
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| 英文摘要: | CBET - 1554078
PI: Yu, Yan
Janus particles are specialized particles whose surfaces have two distinct parts with different surface chemistry on each part. This two-faced feature makes Janus particles especially useful in novel biomedical applications, such as programmed drug delivery, provided the Janus particles can enter cells to carry out their functions. However, effects of the two-faced feature on cellular entry is not well understood. The goals of this CAREER project is to elucidate physical principles governing the entry of Janus particles into cells. Results from this project will provide guidance on how to use surface anisotropy to control the entry of synthetic particles into cells, which has the potential to transform the way multifunctional drug delivery particles are designed. The project will also promote interdisciplinary sciences and education at the interface of chemistry, engineering and biology. A Biomaterials Ambassadors outreach program will be established to bring interdisciplinary sciences to K-12 students and teachers in rural communities in Indiana. This new program will help address the urgent need of improving education in hard-to-reach areas. Visual methods of learning will also be developed to foster critical thinking in undergraduate education.
This CAREER project will focus on ligand-guided cell entry of Janus microparticles. The hypothesis underlying the project is that the surface anisotropy of Janus particles alters the mechanism of cellular entry by changing the interplay between competing forces. To test this hypothesis, dynamics of Janus spheres will be measured with a new single-particle tracking method, which will reveal particle-cell interactions in multi-dimensions. Cellular dynamics, including membrane deformation and actin protrusion, will be measured with high-resolution fluorescence microscopy. The competition between surface anisotropy and shape in controlling the cellular entry pathways will also be investigated with non-spherical Janus particles. The results will establish a direct and quantitative connection between the particle-level anisotropies and the cell-level responses with unprecedented spatial and temporal resolution. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/92852
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| Appears in Collections: | 全球变化的国际研究计划
科学计划与规划
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| Recommended Citation: | |
Yan Yu. CAREER: The Role of Surface Anisotropy in the Cellular Entry of Janus Particles. 2016-01-01.
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