| 项目编号: | 1507384
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| 项目名称: | UNS:Diffuse Optical and Correlation Tomography for Monitoring of Bone-graft Healing |
| 作者: | Regine Choe
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| 承担单位: | University of Rochester
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| 批准年: | 2014
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| 开始日期: | 2015-09-15
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| 结束日期: | 2018-08-31
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| 资助金额: | USD301345
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | re-growth
; tissue
; allograft
; optical method
; live murine autograft model
; diffuse optical tomography
; broken bone
; bone tissue-engineering strategy
; healing damaged tissue
; graft healing
; bone graft
; optical system
; diffuse optical tomographic technique
; comprehensive tomography algorithm
; bone-vascularization monitoring
; deep-tissue longitudinal monitoring
; diffuse correlation tomography
; healing bone
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| 英文摘要: | 1507384
Choe, Regine
The overall goal of this proposal is to develop new and safe imaging methods that use red and infrared light to monitor and image the re-growth of blood vessels in healing bones. These methods are based on diffuse optical tomography (DOT) and diffuse correlation tomography (DCT) as scientific research tools to provide non-invasive, deep-tissue longitudinal monitoring of vascularization of engineered tissues. Tissue engineering is a strategy for using artificial or combinations of natural and artificial materials to assist re-growth of damaged tissues, such as broken bones. The optical methods being developed in this project are based on shining a source of light into the tissue of interest (bone in this case), through the skin, and then analyzing light that is reflected back by the tissues. Techniques that non-invasively monitor and longitudinally assess the vascularization process could significantly accelerate the tissue-engineering field, which will lead to new methods for healing damaged tissues.
Towards this goal, a comprehensive DOT and DCT system will be optimized for monitoring the vascularization of bone grafts. The utility of this approach will be established using well-established murine femoral defect models and bone tissue-engineering strategies. Although there have been various applications of diffuse optical tomographic techniques, their utility in bone-vascularization monitoring, especially in engineered tissue, has never been explored. The proposed research objectives are: (1) Expand an existing optical system into a 3D imaging DOT-DCT system and develop a comprehensive tomography algorithm for longitudinal imaging of murine leg; (2) Compare total hemoglobin concentration from DOT and blood flow from DCT to contrast-enhanced micro-CT and histology and a fluorescent microsphere technique, respectively, using live murine autograft models; (3) Characterize the longitudinal vascular changes due to autografts, devitalized allografts, and allografts with tissue-engineered periosteum using the DOT-DCT system and develop a predictive model for graft healing. Educationally, the proposed research will provide stimulating research topics for graduate and undergraduate students, with opportunities to interact with national and international collaborators in various interdisciplinary fields. The project will also involve educational outreach to underrepresented high school student groups at the University of Rochester. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/93282
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Regine Choe. UNS:Diffuse Optical and Correlation Tomography for Monitoring of Bone-graft Healing. 2014-01-01.
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