项目编号: | 1501764
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项目名称: | DISSERTATION RESEARCH: Shedding Light on the Complex Relationship Between Circadian Clock Variation and the Trade-off Between Flowering Time and Flower Size |
作者: | John Willis
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承担单位: | Duke University
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批准年: | 2014
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开始日期: | 2015-09-01
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结束日期: | 2017-11-30
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资助金额: | USD20634
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资助来源: | US-NSF
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项目类别: | Standard Grant
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国家: | US
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语种: | 英语
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特色学科分类: | Biological Sciences - Environmental Biology
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英文关键词: | trade-off
; flowering time
; period length
; variation
; flower size
; such trade-off
; circadian clock gene network
; large flower
; genetic variation
; different flowering parameter
; important trade-off
; locus
; early flowering
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英文摘要: | A key question in evolutionary biology is how variation is maintained within populations. One possible mechanism is trade-offs between traits. This research will demonstrate whether such trade-offs occur in natural populations of the monkeyflower, Mimulus guttatus, and if developmental patterns influence such trade-offs. For many plants there is a trade-off between flowering time and number or quality of seeds. For example, plants that flower early typically have smaller flowers that are less attractive to pollinators and produce smaller seeds. However, a single population could maintain genetic variation for both early flowering and large flowers because either strategy might be successful. The project also will provide hands-on experience to undergraduates and outreach to middle and high school students.
Mimulus guttatus is a short-lived annual. In both the greenhouse and field there exists a centrally important trade-off between flowering time and flower size. Circadian period length can serve as a highly informative intermediate trait for identifying the genetic underpinnings of flowering time variation. The circadian clock gene network is well characterized, is known to affect flowering time, and varies among populations. The project will identify and characterize loci associated with changes in period length, and determine relationships between period length, differential gene expression, and different flowering parameters. The first aim will be accomplished by determining period length of all members of a pre-existing set of 200 fully sequenced inbred lines generated from a single population. By conducting genome-wide association mapping the researchers will identify loci associated with variation in period length, flowering time, and flower size. The second aim will be accomplished using an RNA sequencing based approach to characterize the contribution of alternate alleles at previously identified loci to differential gene expression. |
资源类型: | 项目
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标识符: | http://119.78.100.158/handle/2HF3EXSE/93419
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Appears in Collections: | 影响、适应和脆弱性 气候减缓与适应
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Recommended Citation: |
John Willis. DISSERTATION RESEARCH: Shedding Light on the Complex Relationship Between Circadian Clock Variation and the Trade-off Between Flowering Time and Flower Size. 2014-01-01.
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