| 项目编号: | 1547790
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| 项目名称: | EAGER: Collaborative Research: Biomanufacturing: Developing a Harvesting Approach for Spatially Targeted Cells from 3D Organoids and Tissues |
| 作者: | Lance Davidson
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| 承担单位: | University of Pittsburgh
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| 批准年: | 2014
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| 开始日期: | 2015-09-01
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| 结束日期: | 2018-08-31
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| 资助金额: | USD150000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | cell
; tissue
; organoid
; biomanufacturing industry
; high throughput cell-harvesting approach
; cellular biomanufacturing operation
; progenitor cell
; cell extraction
; phantom tissue layer-by-layer
; microfluidic approach
; tissue-based therapy
; target cell
; cellular biomanufacturing
; stem cell
; extract live cell
; novel microfluidic harvesting approach
; 3d tissue
; 1547790cellular biomanufacturing
|
| 英文摘要: | PI: LeDuc, Philip R.
Proposal Number: 1547810
PI: Davidson, Lance A.
Proposal Number: 1547790
Cellular biomanufacturing is significant for cell and tissue-based therapies and drug testing applications. Procuring the necessary types of cells from tissues as the starting material is critical for the success of cellular biomanufacturing operations. In this application, the investigators will study a new technology that has been developed for controlling fluid flow at the micrometer scale to extract live cells from living tissues, so they can be used as building blocks for the biomanufacturing industry.
Cells most desired for biomanufacturing grow naturally within a complex heterogeneous three dimensional (3D) environment within human bodies, making their extraction very challenging. The goal of this proposal is to develop high throughput cell-harvesting approaches that allow for the controlled spatiotemporal application of reagents to 3D tissues for targeted cell extraction. To accomplish this, the investigators plan to develop a novel microfluidic harvesting approach to simultaneously image and etch phantom tissues layer-by-layer while recovering target cells downstream; and implement this microfluidics approach for use with laboratory grown organ-buds, i.e. organoids, to enable the extraction of stem cells with spatiotemporal control. The investigators will develop the ability to collect cells from defined locations within small complex 3D tissues such as organoids and use microfluidics to sort extracted cells and maintain their viability. If successful, this project will produce a significant advancement in the targeted harvesting of highly desired stem and progenitor cells from organoids and tissues with a high throughput technology that will then enable the use of recovered cells in cellular biomanufacturing as building blocks for diagnostic and therapeutic applications. The broader impacts of these studies include building an education and training pipeline for preparing future leaders in engineering and science. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/93437
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Lance Davidson. EAGER: Collaborative Research: Biomanufacturing: Developing a Harvesting Approach for Spatially Targeted Cells from 3D Organoids and Tissues. 2014-01-01.
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