项目编号: | 1512616
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项目名称: | UNS: Mechanisms of UV inactivation of viruses for tailored disinfection applications |
作者: | Roberto Rodriguez
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承担单位: | University of Texas Health Science Center Houston
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批准年: | 2014
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开始日期: | 2015-09-01
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结束日期: | 2018-08-31
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资助金额: | USD329997
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资助来源: | US-NSF
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项目类别: | Standard Grant
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国家: | US
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语种: | 英语
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特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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英文关键词: | ultraviolet light
; adenovirus
; uv
; virus disinfection
; disinfection
; virus
; double stranded dna virus
; uv disinfection
; adenovirus inactivation
; uv reactor
; dna repair
; virus inactivation
; adenovirus study
; research
; number
; jc polyomavirus
; disinfection practice
; public health
; inactivation kinetics
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英文摘要: | 1512616 Rodriguez
The goal of this proposal is to gain fundamental insights into the action of ultraviolet light on viruses. The study will examine the molecular level viral responses to ultraviolet irradiation to improve current ultraviolet light disinfection and design of future ultraviolet light technologies. The research aims to answer a number of fundamental questions around virus inactivation with ultraviolet light that can inform engineering practice, public health, and subsequent regulatory policy and thus has broad reaching impacts.
The proposed activity will advance knowledge in a number of key areas. New fundamental knowledge will be developed into the molecular-level response of viruses to ultraviolet light irradiation during disinfection practice. Specifically, in addition to adenoviruses, new studies will be performed on another double stranded DNA virus - JC polyomavirus - to test the generalizability of the findings with adenoviruses in response to low-pressure and medium-pressure ultraviolet light. This research will move beyond the traditional evaluations of laboratory strains of adenoviruses and isolate and test environmental strains to determine if there are any differences in the ultraviolet light -response that may be related to acclimated responses (such as DNA repair) from repeated exposure to and propagation in laboratory cell culture lines. A number of new areas in molecular microbiology methods, some of which we have developed, will be exploited including early stage infection inhibition due to specific ultraviolet light wavelengths, DNA repair from trans-lesion synthesis, protein damage assessment using electrophoresis and mass spectrometry techniques, and DNA damage using a new long range PCR technique we recently published. These fundamental findings will be used to develop a tailored wavelength ultraviolet-LED based energy efficient irradiation system to optimize disinfection at lower cost than conventional ultraviolet sources. The work proposed herein will: (1) evaluate inactivation kinetics and wavelength sensitivity of two double stranded DNA viruses, (2) extend the adenovirus studies to adenoviruses isolated from the environment, (3) evaluate the fundamental role of DNA repair in the cell culture infectivity outcomes, (4) incorporate newly developed molecular biology-based assays to assess DNA damage and protein damage from ultraviolet light irradiation at varying wavelengths, and, (5) understand the implications for disinfection using tailored wavelength approaches made possible by the development of nanometer-specific ultraviolet light emitting diodes (LEDs). Regulators and public health officials need information to help make sound decisions for the public sector. The issue of virus disinfection for small systems is currently contentious as the Groundwater Rule specifically forbids the use of UV for any virus inactivation credit due to the high doses required for adenovirus inactivation that cannot currently be field-validated in UV reactors. Therefore, the timing of this research is critical if UV disinfection is going to play any role for virus disinfection in groundwater. Thus, small-systems wanting to use UV for protection of public health, may needlessly suffer. |
资源类型: | 项目
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标识符: | http://119.78.100.158/handle/2HF3EXSE/93572
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Appears in Collections: | 影响、适应和脆弱性 气候减缓与适应
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Recommended Citation: |
Roberto Rodriguez. UNS: Mechanisms of UV inactivation of viruses for tailored disinfection applications. 2014-01-01.
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