| 项目编号: | 1457440
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| 项目名称: | Collaborative Research: Transitions and Transcriptomics: a Novel Approach to Understanding Shifts in Floral Developmental Pathways Preceding the Origin of the Pentapetalae |
| 作者: | Dianella Howarth
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| 承担单位: | Saint John'
; s University
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| 批准年: | 2014
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| 开始日期: | 2015-07-15
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| 结束日期: | 2018-06-30
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| 资助金额: | USD367284
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Biological Sciences - Environmental Biology
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| 英文关键词: | pentapetalae
; shift
; flowering plant
; research
; typical pentapetalae flower
; hybridization approach
; other researcher
; pentapetalae flower
; pentapetalae clade
|
| 英文摘要: | Flowering plants are the most abundant and diverse form of plant life on Earth. This dominance is largely due to the evolutionary success of one group, the Pentapetalae, characterized by whorled flower parts in multiples of five, which account for over 70% of described species. In contrast, flower parts are arranged in multiples of three (monocots, for example), two, or a variable number in less abundant groups of flowering plants. The shift to whorled pentamery of the Pentapetalae occurred about 100 million years ago, and appears to have been one of the most successful evolutionary innovations of not only flowering plants, but of green plants in general. This research will investigate the evolution of the underlying genetic changes that contributed to this ancient evolutionary event. It promises to discover the new and/or modified genetic pathways that produced the typical Pentapetalae flower, one of the major mysteries of plant evolution. Several undergraduates will be trained in phylogenetic and molecular biology methods and analyses, and high school teachers will participate in the research through a summer workshop. Data generated will be shared freely online and will provide tools for other researchers and the public to investigate other questions about flowering plant evolution.
The researchers will sequence genes expressed during flower development in a phylogenetically representative sample of flowering plants and employ state-of-the-art transcriptome analysis techniques to achieve three major goals. Firstly, phylogenetic relationships among lineages subtending the Pentapetalae clade will be reconstructed. Robustly resolved phylogenetic relationships of the lineages preceding the shift to the pentamerous whorled flower are currently lacking but pivotal to interpreting the evolutionary sequence of genetic changes that underlie this key morphological innovation. Secondly, several comparative methods will be used to identify, date, and determine the evolutionary impact of whole-genome and/or gene duplication event(s) that predate the origin of the Pentapetalae. The occurrence of whole-genome duplication events prior to Pentapetalae is widely acknowledged but their precise positions are uncertain. Lastly, RNA-seq and in situ hybridization approaches will be used to infer spatial patterns of gene expression in non-Pentapetalae versus Pentapetalae flowers to identify gene expression changes that correlate with a shift to whorled pentamery in the latter. Together, this integration of approaches will provide exciting new insights into our understanding of flowering plant evolution |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/93993
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Dianella Howarth. Collaborative Research: Transitions and Transcriptomics: a Novel Approach to Understanding Shifts in Floral Developmental Pathways Preceding the Origin of the Pentapetalae. 2014-01-01.
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