| 项目编号: | 1510028
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| 项目名称: | UNS: Intracellular Drug Delivery Mediated by Laser-activated Nanoparticles |
| 作者: | Mark Prausnitz
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| 承担单位: | Georgia Tech Research Corporation
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| 批准年: | 2014
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| 开始日期: | 2015-07-01
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| 结束日期: | 2018-06-30
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| 资助金额: | USD329011
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | nanoparticle
; drug molecule
; nanoparticle temperature
; cell
; laser-activated nanoparticle
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| 英文摘要: | CBET - 1510028
PI: Prausnitz, Mark
This project will explore a novel way to make cell membranes temporarily permeable so that drug molecules are able to enter the cell and carry out their therapeutic functions. The method relies on irradiating nanoparticles interspersed between the cells with pulsed laser energy. Preliminary data show that irradiating the nanoparticles leads to a significant increase in the cellular uptake of a fluorescent molecule without loss of cell viability. The research could lead to a new way to enhance the delivery of biomolecules to targeted cells for therapeutic, diagnostic and laboratory applications.
This project will test the hypothesis that the laser energy raises the nanoparticle temperature and vaporizes surrounding liquid, producing local stress fields that can temporarily and non-destructively permeabilize cell membranes, thereby allowing intracellular uptake of exogenous molecules by living cells. A series of experiments will quantify the spatio-temporal characteristics of the forms of energy released by laser-activated nanoparticles to test the hypothesis that the dominant characteristic of energy transduction by a nanoparticle is the rapid generation of an enveloping vapor bubble that condenses slowly after formation. The research also will determine which features of the particle transduction outputs correlate to high intracellular uptake and cell viability to test the hypothesis that cellular bioeffects are controlled by acoustic and thermal effects occurring on the scale of a cell diameter. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/94300
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Mark Prausnitz. UNS: Intracellular Drug Delivery Mediated by Laser-activated Nanoparticles. 2014-01-01.
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