| 项目编号: | 1512157
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| 项目名称: | UNS:Photomodulation of Forster Cycle in a Fluorescent Protein |
| 作者: | Kaan Kalkan
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| 承担单位: | Oklahoma State University
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| 批准年: | 2014
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| 开始日期: | 2015-07-01
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| 结束日期: | 2018-06-30
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| 资助金额: | USD106578
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | forster cycle
; photomodulation
; fluorescent protein
; green fluorescent protein
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| 英文摘要: | PI: Kalkan, Kaan
Proposal No.: 1512157
This project seeks to develop new types of optical probes that are faster and more efficient at switching, while reducing effects of photobleaching. They will study the photophysics of energy transfer between metal nanoparticles and fluorescent proteins to develop stable photoactivatable probes for super-resolution microscopy applications.
The hypothesized photoswitching is based on photocreation of the intermediate state in the Forster cycle of the FP using surface-enhanced optical pumping. This photomodulation of the Forster cycle will be verified and studied by single molecule surface-enhanced Raman scattering and micro-optical spectroscopy. This effort will develop a photoswitch using Green Fluorescent Protein (GFP) - silver NP conjugates as its prototype. The photomodulation is based upon photopopulation of the intermediate form (I-form) of GFP by excited state proton transfer that red-shifts the optical absorption from 395 to 490 nm. This spectral shift in absorption, in turn, is expected to modulate the Mie scattering of the plasmonic NP by resonant energy transfer to the FP. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/94319
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Kaan Kalkan. UNS:Photomodulation of Forster Cycle in a Fluorescent Protein. 2014-01-01.
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