| 项目编号: | 1500654
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| 项目名称: | DISSERTATION RESEARCH: Identifying the pollen tube chemoattractant regulating gamete recognition in the wild tomato clade (Solanum sect. Lycopersicon) |
| 作者: | Leonie Moyle
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| 承担单位: | Indiana University
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| 批准年: | 2014
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| 开始日期: | 2015-06-01
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| 结束日期: | 2016-11-30
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| 资助金额: | USD20129
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Biological Sciences - Environmental Biology
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| 英文关键词: | female gamete
; domesticated tomato
; research
; species
; male gamete
; gamete recognition
; gamete attraction
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| 英文摘要: | This study aims to understand gamete recognition (chemical cues used by male and female gametes to recognize and attract each other) as a mechanism of sexual reproduction, and how it acts to prevent reproductive events between different species. This work uses the domesticated tomato and its twelve diverse wild species relatives, which vary in many reproductive and functional traits. The project will first identify the chemical signal derived from the female gamete that attracts the male gamete within species. Second, it will characterize variation in this signal across species and connect this variation to previously observed reduced gamete attraction between species. In addition to understanding molecular mechanisms of normal and attenuated reproductive function, these data could aid in overcoming breeding barriers between domesticated tomato and its genetically diverse wild relatives. This project will include research by undergraduates from underrepresented groups and potential insights of agricultural importance for crop breeding.
To identify the female gamete-derived chemoattractant and its specific tissue of origin, this project will use high depth nucleotide sequencing technologies (RNA-seq) for tissue specific expression analysis. It will assess molecular divergence of this signaling protein via variation in sequence and/or expression across 13 closely related species. These combined approaches will explicitly link patterns of molecular expression and DNA variation to functional divergence in reproductive behaviors. Although many studies have demonstrated that species show reduced sexual compatibility, the underlying molecular mechanisms are generally unknown. Together, this research will provide an unprecedented opportunity to examine the patterns and consequences of evolutionary changes in a reproductive molecule, including for the development of new species. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/94499
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Leonie Moyle. DISSERTATION RESEARCH: Identifying the pollen tube chemoattractant regulating gamete recognition in the wild tomato clade (Solanum sect. Lycopersicon). 2014-01-01.
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