| 项目编号: | 1509106
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| 项目名称: | UNS: Development of a Micropost Approach for the Contractile Maturation of iPS-Derived Cardiomyocytes |
| 作者: | Nathan Sniadecki
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| 承担单位: | University of Washington
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| 批准年: | 2014
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| 开始日期: | 2015-06-01
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| 结束日期: | 2018-05-31
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| 资助金额: | USD454800
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | hipsc-cm
; project
; cardiomyocyte
; mechanical cue
; effective approach
; heart development
; contractile power
; flexible micropost
; developmental study
; developmental biology
; career development
; maturation marker
; new cardiomyocyte
; contractile force
; cardiac development
; cardiac maturation
|
| 英文摘要: | PI: Sniadecki, Nathan J.
Proposal Number: 1509106
The goal of this project is to develop a novel strategy that improves the functionality of cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs) in culture. Human induced pluripotent stem cells are not harvested from embryos, which means they can serve as a noncontroversial and plentiful source of cardiomyocytes. These cells have the potential to be used to reverse the damage to cardiac tissue caused by heart attacks, assess new pharmacological treatments for heart disease, and serve as a model system for studying heart development. To unlock the potential of hiPSC-CMs, this project will evaluate the effects of mechanical cues like substrate stiffness, cell shape, cyclic strain, and cell-cell contact in combination with biochemical treatments that promote cardiac maturation. The expected outcome of this project is an effective approach for in vitro culture of hiPSC-CMs that can be widely adopted.
The lack the contractile power and maturation markers for hiPSC-CMs is a major challenge to overcome in order for these cells to be used effectively as a source of new cardiomyocytes. To address this challenge, the efforts in this project are centered on using arrays of flexible microposts for evaluating the contractile forces of hiPSC-CMs and quantitative fluorescence microscopy techniques to assess their myofibril structure, hypertrophy, isoform expression, and calcium handling. Tasks 1 and 2 of this project focus on studying the mechanical cues that improve the contractility and maturation of hiPSC-CMs, including cell alignment, cell-cell contact, extracellular stiffness, and applied strain. Task 3 examines the effectiveness of these mechanical cues alongside biochemical treatments that are related to cardiac development. The broader impacts of this work lie in the potential advancement of new cardiac therapies, drug screening, and developmental studies. In addition, the project has educational and outreach components that will impact the training and career development of future engineers who will work at the interface between engineering, cardiology, and developmental biology. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/94588
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Nathan Sniadecki. UNS: Development of a Micropost Approach for the Contractile Maturation of iPS-Derived Cardiomyocytes. 2014-01-01.
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