| 项目编号: | 1450829
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| 项目名称: | BRAIN EAGER: Spatially-Resolved In Vivo Optogenetic Stimulation and Imaging Platform |
| 作者: | Stephen Boppart
|
| 承担单位: | University of Illinois at Urbana-Champaign
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| 批准年: | 2013
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| 开始日期: | 2014-09-01
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| 结束日期: | 2016-12-31
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| 资助金额: | USD300000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | imaging fiber bundle
; imaging
; neural circuit
; brain eager
; spatially-resolved optogenetic stimulation
; optogenetic application
; spatially-controlled optical stimulation
; imaging platformsignificancethe successful outcome
; control
; biomedical imagingcourse
; technical descriptionoptogenetics
; new imaging
; k-12
; vivo optogenetic stimulation
; optogenetic stimulation
; optogenetically-modified neuron
; eager project
|
| 英文摘要: | PI: Boppart, Stephen A.
Proposal: 1450829
Title: BRAIN EAGER: Spatially-Resolved In Vivo Optogenetic Stimulation and Imaging Platform
Significance
The successful outcome of this research project will have a broad impact in neuroscience in addition to optical science and engineering. The PI will use implanted imaging fiber bundles that will enable
in vivo imaging as well as spatially-controlled optical stimulation and optical feedback of
large-area neural circuits. Current fibers only indiscriminately illuminate large-areas. Optogenetics is expected to make a broad impact in neuroscience, as well as medical science and clinical medicine in the future. This proposed research offers the potential to have an even greater impact by controlling the light stimulus and enhancing specificity in the control of neural circuits. The results of this project will be shared widely amongst the scientific and engineering communities, and also across wide segments of society in outreach activities. The new imaging and visualization capabilities will inspire K-12 students to think about how technology can be used to see things one cannot normally see, and how we can invent new ways of seeing the world around us and discovering new knowledge. Outreach activities will include demos of these imaging fiber bundles and novel light sources to K-12 and community groups through
annual Engineering Open House events, as well as integration of these technological methods in Prof. Boppart?s undergraduate ECE/BioE 467 Biophotonics and ECE/BioE 380 Biomedical Imaging
courses.
Technical Description
Optogenetics is a rapidly developing field with an ever-expanding toolkit of molecular biology
techniques to enable light-activated switching and control of cells, most commonly neurons.
Equally significant advances have occurred in optical science and engineering. By understanding
and exploiting physics-based principles of how light interacts in photonic crystal fibers (PCFs) and within imaging fiber bundles, it is possible to generate, control, and optimize a wide range of new optical parameters for in vivo optogenetic stimulation. Traditionally in in vivo optogenetic applications, light has been sent down single multi-mode optical fibers to diffusely illuminate the brain, relying on the molecular biology of optogenetically-modified neurons for cell and circuit specificity. This EAGER project will uniquely develop and demonstrate the use of imaging fiber bundles, and the generation of specific light pulse parameters to enable spatially-resolved optogenetic stimulation and imaging of neural circuits in vivo. These novel neurotechnologies will enable new investigations underlying behavior and cognition. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/95531
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Stephen Boppart. BRAIN EAGER: Spatially-Resolved In Vivo Optogenetic Stimulation and Imaging Platform. 2013-01-01.
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