| 项目编号: | 1438340
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| 项目名称: | Collaborative proposal: Developing a battery of methods for the study of the trafficking mechanisms and transformations of silver and gold nanomaterials in human cells |
| 作者: | Ioana Sizemore
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| 承担单位: | Wright State University
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| 批准年: | 2013
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| 开始日期: | 2014-09-01
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| 结束日期: | 2018-08-31
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| 资助金额: | USD225000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | method
; trafficking mechanism
; human cell
; study
; battery
; transformation
; nanomaterial shape
; cellular trafficking
; collaborative proposal
; silver nanoparticle
; relevant gold nanorod
; cell behavior
; gold nanomaterial
; cellular exposure
; relevant gold nanoparticle
; representative silver nanoparticle
; interdisciplinary method
; zerdaproposal number
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| 英文摘要: | PI: Ioana Pavel Sizemore/Adam de la Zerda
Proposal Number: 1438340/1434465
Institution: Wright State University/Stanford University
Title: Collaborative proposal: Developing a battery of methods for the study of the trafficking mechanisms and transformations of silver and gold nanomaterials in human cells
The extensive use of silver nanoparticles (AgNPs) in consumer products and industry and the use of many kinds of NPs in healthcare as diagnostic and therapeutic tools require an understanding of their potential toxicities. However, as observed by the National Nanotechnology Initiative, there are only a limited number of techniques that could expand our understanding of the mechanisms by which nanomaterials influence cell behavior. The main scientific goal of this project is to fill these knowledge gaps by developing a battery of tools for the study of the trafficking mechanisms and transformations of environmentally representative AgNPs and biomedically relevant gold nanorods (AuNRs) in human cells. Key parameters, which will be considered in order to test the efficiency and practicality of the proposed methods, will include nanomaterial shape, surface charge, dose, and exposure time. The methods that are to be developed will be captured and shared through Standard Operating Procedures (SOPs). In addition, the knowledge gained from the proposed research will be used in setting up new course modules and will be disseminated into the broader community, e.g., through interactions with a local museum and development of two hour hands-on laboratory modules for over 100 K-6 through K-9 students.
A battery of interdisciplinary methods (ICP-OES/TFF, Raman-SERS/MatLab codes, and electron microscopy /bioassays) will be used for the study of the trafficking mechanisms and transformations of environmentally representative silver nanoparticles (AgNPs) and biomedically relevant gold nanoparticles (AuNRs) in human cells. Key parameters that will be considered to test the efficiency and practicality of the proposed methods will include nanomaterial shape, surface charge, dose, and exposure time. To accomplish their goals, The PIs propose two specific aims. In Aim1, the PIs will characterize the physico-chemical properties of NPs, according to the U.S. EPA standards, before cellular exposure and then at various benchmark times during cellular trafficking. In Aim 2, using their suite of tools, the investigators will study the trafficking mechanisms of AgNPs and AuNRs in human cells as a function of nanomaterial shape, surface charge, dose, and exposure time. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/95565
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Ioana Sizemore. Collaborative proposal: Developing a battery of methods for the study of the trafficking mechanisms and transformations of silver and gold nanomaterials in human cells. 2013-01-01.
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