| 项目编号: | 1403053
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| 项目名称: | COLLABORATIVE RESEARCH: Polypeptide-Templated Nanoparticles as Colorimetric Sensors of Ionizing Radiation |
| 作者: | Jeffrey Heys
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| 承担单位: | Montana State University
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| 批准年: | 2013
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| 开始日期: | 2014-09-01
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| 结束日期: | 2017-08-31
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| 资助金额: | USD100430
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | radiation
; amino acid
; x-ray
; research
; polypeptide-based colorimetric nanobiosensor
; novel radiation-sensing method
; colorimetric sensor
; polypeptide-templated nanoparticles
; templated nanoparticle
; colorless metal salt
|
| 英文摘要: | Proposal: 1403860/1403053
PI: Rege/Hayes
Institution: Arizona State University/ Montana State
Title: Polypeptide-Templated Nanoparticles as Colorimetric Sensors of Ionizing Radiation
Ionizing radiation, including X-rays and gamma rays, is useful in several medical, defense, space, and agricultural applications. However, uncontrolled ionizing radiation can lead to large scale disasters and damage to human health. Given the necessity to balance potential deleterious effects and widespread application, it is important to develop novel radiation-sensing methods in order to ensure safety and prevent accidents. We have recently developed a novel nanobiosensing approach in which we employed proteins for templating the formation of metal nanoparticles from colorless metal salts. This change from colorless metal salts to colored dispersions of nanoparticles in water is a simple and visual indicator of ionizing radiation. In the proposed research, we will carry out a fundamental study on how different amino acids and small peptides mediate nanoparticle formation in the presence of different doses of ionizing radiation. Both, experimental and mathematical modeling approaches will be employed to study this behavior.
Our preliminary results indicated that polypeptides are capable of templating plasmonic metal nanoparticles from colorless metal salts in presence of ionizing radiation (X-Rays). In the proposed research, we will carry out an extensive investigation into how each of the 20 natural amino acids template bimetallic gold-silver nanoparticles upon exposure to different doses of ionizing radiation. We will then investigate dipeptides (?minimal peptides?), in which an amino acid with activity for templating the formation of plasmonic nanoparticles is juxtaposed with an amino acid that can bind and stabilize the templated nanoparticles. In addition, pentapeptides in which the binding and templating amino acids are separated by a tri-glycine linker will also be investigated. Mathematical modeling will be employed in order to investigate how different amino acids and peptides, together with experimental physicochemical conditions influence the formation kinetics of bimetallic nanoparticles. Water radiolysis, metal ion reduction, and nanoparticle formation will be modeled, and effects of pH, scavenging agents, and templating biomolecules will be investigated. Experimental and modeling studies will be employed for molecular engineering and developing sequence-property relationships for polypeptide-based colorimetric nanobiosensors for ionizing radiation. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/95678
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Jeffrey Heys. COLLABORATIVE RESEARCH: Polypeptide-Templated Nanoparticles as Colorimetric Sensors of Ionizing Radiation. 2013-01-01.
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