| 项目编号: | 1451319
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| 项目名称: | EAGER: Building a Framework to Advance Research through Undergraduate Participation: Investigating pH in 3D Tissue Test Systems |
| 作者: | Karen Burg
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| 承担单位: | Clemson University
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| 批准年: | 2013
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| 开始日期: | 2014-09-01
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| 结束日期: | 2018-02-28
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| 资助金额: | USD69885
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | tissue
; research
; layer
; undergraduate
; ph shift
; ph
; conventional 3d tissue system
; advance research
; research result
; innovative research program
; model tissue
; overall tissue function
; ph map
; 3d tissue test systemsthis eager award
; dimensional tissue
; previous research experience
; team-based undergraduate research opportunity
; tissue system application
; wide-scale research participation
; heterogeneous 3d bench-top system
; pi
; undergraduate research
; tissue construction
; undergraduate participation
; current undergraduate research course
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| 英文摘要: | Proposal: 1451319
PI: Burg, Karen
Title: EAGER: Building a Framework to Advance Research through Undergraduate Participation: Investigating pH in 3D Tissue Test Systems
This EAGER award will lead to the development of methods for both constructing complex three dimensional tissues, but also deconstructing the tissues after use such that each layer of the tissue can be analyzed. Developing new techniques for tissue construction that allow facile layer by layer deconstruction or dis-assembly of tissues will allow investigators to ask for the first time how the role of spatial gradients of chemicals, along with cell to cell contacts, impacts the survival and function of individual cells within the tissue and eventually overall tissue function. Along with this innovative research program, the PI will also develop novel mentoring strategies for undergraduates engaged in research. She poses some unconventional hypotheses about a fused research and mentoring methodology which she believes will lead to greater retention of diverse students in STEM fields. With funds from this award, the PI aims to develop a scalable model of fused research and mentoring that will potentially have a high impact on undergraduate research and training, and retention of students engaged in such activities.
The overarching theme of the proposed work is to quantify and evaluate the role of pH in tissue system applications. To this end, a novel, heterogeneous 3D bench-top system will be developed to model tissue engineered breast and bone. Unlike conventional 3D tissue systems, the breast model will incorporate stacked layers with "peel-able" design, in which each layer has specific characteristics. This design is also unique in that the model may be easily taken apart and analyzed. The orthopedic model will incorporate gel and a ceramic bone graft filler and will explore acidic to basic ranges of pH shift. The breast model will incorporate gel and polymeric beads and will explore neutral to acidic ranges of pH shift. Following culture of either model, the system will be deconstructed, layer by layer, and pH test liquid applied to spots across each paper. In this manner a pH map will be constructed. If successful, the stacking approach will allow, for the first time, mapping of the interior of tissue engineered systems. At the same time the PI will build on a previous Research Experience for Minorities (REM) program to test new methods to provide critical thinking skills to undergraduates engaged in research. She will use a scientific approach to gathering and analyzing data related to the efficacy of current undergraduate research courses and a team-based undergraduate research opportunity as the means to teach critical thinking through research. The results of this study will help provide guidance as to where limited university resources should be applied to provide wide-scale research participation by undergraduates and maximize research results while addressing the need to improve the critical thinking skills of the participants. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/95745
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Karen Burg. EAGER: Building a Framework to Advance Research through Undergraduate Participation: Investigating pH in 3D Tissue Test Systems. 2013-01-01.
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