| 项目编号: | 1452290
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| 项目名称: | EAGER: Engineering bacterial lectins for glycomics analysis |
| 作者: | Ruizhen Chen
|
| 承担单位: | Georgia Tech Research Corporation
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| 批准年: | 2013
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| 开始日期: | 2014-09-01
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| 结束日期: | 2015-08-31
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| 资助金额: | USD40362
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | glycomic analysis
; glycan
; lectin molecule
; analysis format
; pi
; research
|
| 英文摘要: | 1452290
Chen
Georgia Tech
Glycosylation is the process that attaches glycans to proteins, lipids, or other organic molecules. Glycans on proteins and lipids may serve as markers or identifiers in many vital biological processes such as cell growth, cell-cell interactions,and cell mig. Unusual glycosylation has been implicated in many different types of diseases including cancer. The lack of simple and effective tools to detect glycolsylation creates a significant obstacle to the advances in this area. The PI proposes a study to develop new tools and methods useful to assist in the detection of glycolsylation that are low cost and simple to use.
Two specific aims are proposed:
Aim 1: the PI proposes to develop and validate a novel glycoprotein quantification method, which can be incorporated into commonly used protein separation techniques. If successful the work proposed in could extend the use of 1-D and 2-D electrophoresis separation techniques. It could also make these tools more reliably employed and useful tool for glycoscience.
Aim 2: As an example the PI proposes to demonstrate that the developed methods could be used for a real-time label-free bacterial surface glycome profiling and for study of dynamics of bacterial glycosylation in response to environmental conditions.
The proposal also plans to incorporate engineered lectin molecules into the common protein separation and analysis format, such as SDS gel electrophoresis. If successful, this will change how glycans are detected by eliminating the need for antibody and other expensive reagents commonly in the processes.
The proposed research may advance the development of much needed tools to simplify methods commonly used in glycoscience research. The success of the research has the potential to make glycomic analysis less arduous and less time consuming, less labor and material intensive. As a result, glycomic analysis may no longer be conducted by only a small number of specialized laboratories and may become much more accessible to a common laboratory. Thus the research will have significant impact on the field by bringing low cost tools to glycoscience researchers. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/95749
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Ruizhen Chen. EAGER: Engineering bacterial lectins for glycomics analysis. 2013-01-01.
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