| 项目编号: | 1402712
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| 项目名称: | CELL INTERFACIAL ENGINEERING WITH SOFT LbL SHELLS |
| 作者: | Vladimir Tsukruk
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| 承担单位: | Georgia Tech Research Corporation
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| 批准年: | 2013
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| 开始日期: | 2014-08-01
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| 结束日期: | 2017-07-31
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| 资助金额: | USD330000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | cell
; bacterial cell
; cell-based
; shell
; proposal
; soft lbl shellsbiological cell
; protective shell
; lbl polymer
; interfacial organization
; cell-based therapy
; cell-based biosensing
; cell surface
; microbial cell
; cellular technology
; cell interfacial
|
| 英文摘要: | PI: Tsukruk, Vladimir
Proposal Number: 1402712
Institution: Georgia Tech Research Corp
Title: CELL INTERFACIAL ENGINEERING WITH SOFT LbL SHELLS
Biological cells are complex machineries that are able to do many things that are still beyond the capabilities of engineering and science. Those capabilities can be used by preserving the cells in a protective shell before bringing them into a hostile environment, where the cell's functionalities are to be used. This proposal presents a new way to construct responsive coatings for cells, such as yeast and bacterial cells, using layer-by-layer (LbL) techniques to make polymeric capsules based on hydrogen bonding of the polymeric layers, particularly silk-based polymers. This proposed approach will bypass the known toxicity of past cationic (positively charged) LbL polymers, as well as resolve some of the issues with silica sol-gel and hydrogel encapsulation technologies. The encapsulation methodology described in this proposal, if successfully developed, could lead to a high impact on new ways to protect cells from their environments and thus enable cellular technologies in cell-based biosensing, cell-based therapies, and tissue engineering.
The proposed approach is systematic and well laid out, involving identification of components from which to fabricate the multilayers, studies of the transport properties of the multilayers, and then encapsulation of yeast and bacterial cells with the select polymers and investigation of protective effects of the shells on the cells as they are challenged in various environments. Novel aspects of the proposal include the use of shear and/or ionic strength changes into induce conformational changes (beta-sheet formation) in silk-based proteins to achieve physical cross-linking. The planned approach of using soft bio-interfaces that are robust, semipermeable, and cyto-friendly will help the investigators to reach their ultimate goal of gaining an understanding of interfacial organization, transport properties, and the mechanism of interactions between the shells and cell surfaces that are necessary to preserve the viability and function of genetically engineered microbial cells. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/96079
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Vladimir Tsukruk. CELL INTERFACIAL ENGINEERING WITH SOFT LbL SHELLS. 2013-01-01.
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