| 项目编号: | 1402051
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| 项目名称: | DISSERTATION RESEARCH: Is richer always better? Testing the biotic resistance hypothesis in ornamental fish microbiomes |
| 作者: | Linda Amaral Zettler
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| 承担单位: | Marine Biological Laboratory
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| 批准年: | 2013
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| 开始日期: | 2014-07-01
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| 结束日期: | 2016-06-30
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| 资助金额: | USD20865
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Biological Sciences - Environmental Biology
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| 英文关键词: | biotic resistance
; microbiome
; fish microbiome
; researcher
; research
; disease resistance
; antibiotic
; antibiotic treatment
; fish
; host
; role microbiome
; laboratory research
; ornamental fish shipment
; microbiome richness
; known fish pathogen
; ornamental fish industry member
|
| 英文摘要: | The sum of microorganisms living on and in a host is called the "microbiome". A host's microbiome often has more cells and functions than the host itself, and plays important roles in nutrition, health and disease. Antibiotic treatment disrupts microbiomes in mice and humans and increases the likelihood of bacterial infections after treatment. Fish depend on their microbiome and are often treated with antibiotics in commercial settings, particularly along ornamental trade routes where suppliers must deliver a disease-free product to retail pet shops. This study will test if antibiotics have the unintended consequence of disturbing fish microbiomes during shipment, thereby reducing disease resistance after fish have arrived in retail store tanks. The researchers will collaborate with the Woods Hole Partnership in Education Program to involve underrepresented groups in the laboratory research. Additionally, the researchers will continue ongoing collaborations with ornamental fish industry members, engaging the private sector in science-based solutions to disease. This research will provide valuable insight into the role microbiomes play in aquaculture diseases and potential probiotic treatments, and contribute to the advancement of science-based solutions to aquaculture development.
A reduction in biotic resistance, or the ability to protect the host from colonization and
proliferation of external pathogens, often accompanies antibiotic treatments. The first aim of this research is to assess the influence of antibiotics on disease resistance in fish microbiomes, using aquarium-based experiments in the laboratory. Ornamental fish shipments with and without the use of antibiotics will be simulated, followed by a spike of a known fish pathogen. Biotic resistance will be measured by mortality, and the relative abundance of the spiked pathogen within fish microbiomes. In humans and mouse models, re-inoculation after antibiotic treatment with a high-richness microbial community restores biotic resistance. The second objective of this research will be to test a range of bacterial inoculates for their ability to restore biotic resistance in fish microbiomes after antibiotic treatment. These inoculates will vary in their richness and composition, and biotic resistance will be assessed as above, alongside resulting microbiome richness. The researchers will test the hypothesis that a low-richness recovery inoculum of probiotic species representing a variety of disease resistance mechanisms will provide the strongest possible microbiome-mediated biotic resistance. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/96493
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Linda Amaral Zettler. DISSERTATION RESEARCH: Is richer always better? Testing the biotic resistance hypothesis in ornamental fish microbiomes. 2013-01-01.
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