| 项目编号: | 1401237
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| 项目名称: | DISSERTATION RESEARCH: Validation of positive selection on gene regulation at the genome-wide scale |
| 作者: | Pardis Sabeti
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| 承担单位: | Harvard University
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| 批准年: | 2013
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| 开始日期: | 2014-07-01
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| 结束日期: | 2017-06-30
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| 资助金额: | USD21822
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Biological Sciences - Environmental Biology
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| 英文关键词: | positive selection
; research
; variant
; high-throughput validation
; causal variant
; genetic diversity
; mirsnp
; high-throughput
; regulatory variant
; low-throughput validation
|
| 英文摘要: | Variation in the regulation of the genes in human DNA is thought to have played a critical role in the evolution of organism complexity, and thus the characterization of regulatory variants selected for in the human genome is broadly informative to understanding the evolution of multicellular, complex organisms as a whole. The direct product of this research is the identification of variants that may lead to population-specific phenotypes, such as resistance to endemic infectious threats, adaptation to high sun exposure or high altitude, or heightened sensory function in situations of predation. Follow-up work of the variants identified could include field studies in Asia and Africa that will promote the development of scientific infrastructure in the developing world. Because this research will require close partnerships with both computational and experimental biologists it will initiate productive scientific collaborations between these synergistic fields of evolutionary biology.
Positive, or Darwinian, selection is an evolutionary force driving the creation of genetic diversity that enhances the survival and/or reproductive fitness of an organism. The availability of human whole-genome sequences marks an exciting time in which signatures of positive selection can be detected directly from primary genomic sequence without bias for the biological functions affected or the molecular mechanisms involved. Because tests for positive selection are limited by linkage disequilibrium of the causal variant with many non-causal variants which "hitchhike" along the selective sweep, the Composite of Multiple Signals (CMS) test was recently developed. The CMS test identifies genomic regions under positive selection with 100-fold improved resolution and when applied to human whole-genome sequencing data, 90% of causal variants were predicted to be regulatory. This is intriguing because most examples of positive selection identified to date involve coding regions, and positive selection on regulatory variants remains largely unchartered territory. One class of regulatory variants is single-nucleotide polymorphisms in miRNA binding sites (mirSNPs). Based on preliminary prediction in silico and low-throughput validation of several mirSNPs identified by CMS, this project will use a high-throughput method to test all mirSNPs residing within CMS regions by functional analysis with a massively parallel reporter assay. By combining in silico prediction with high-throughput validation, the research will drastically improve the ability to detect mirSNPs which have driven recent positive selection in the human lineage. High-throughput validation allows the comparison of multiple linked variants in tandem, providing a more direct assessment of which variant(s) likely contributed to the selective sweep. This study will provide much-needed insight into the impact of positive selective forces on regulatory variation, particularly variation in miRNA binding sites, and in addition to uncovering the causal allele within CMS regions, the project will also increase understanding of the role this distinct functional class of regulatory elements plays in adaptive evolution. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/96497
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Pardis Sabeti. DISSERTATION RESEARCH: Validation of positive selection on gene regulation at the genome-wide scale. 2013-01-01.
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