| 项目编号: | 1403495
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| 项目名称: | Hormone-Responsive Electrodes for Quantitative Discourse with Endocrine Cells |
| 作者: | Christopher Easley
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| 承担单位: | Auburn University
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| 批准年: | 2013
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| 开始日期: | 2014-07-01
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| 结束日期: | 2018-06-30
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| 资助金额: | USD329990
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | endocrine cell
; hormone-responsive electrode
; cell culture
; protein
; quantitative discourse
; hormone-responsive
; cell culture system
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| 英文摘要: | Proposal Number: 1403495
Principal Investigator: Christopher J. Easley
Title: Hormone-Responsive Electrodes for Quantitative Discourse with Endocrine Cells
General
Biological systems communicate using cascades of molecular signals, exploiting numerous feedback control loops similar to automobile cruise control. To aid our understanding of these systems, there remains a need for materials that respond to biological signaling molecules such as proteins and DNA. We have developed measurement techniques (proximity assays) that can respond to very low levels of proteins, translate this signal into controlled DNA binding, then communicate the signal to an electrode for recording protein quantity. These techniques can also release DNA strands in proportional amounts to input proteins. The main objective of this proposal is to show that our surface-based proximity assays can sense hormone levels secreted by endocrine cells, then respond proportionally by releasing agents that modify secretion. Upon completion, we will have developed a tunable "cruise control" system for hormone secretion, one that should allow cell culture systems in the laboratory to more closely mimic their natural biological environment.
Technical
Our recently developed proximity-based protein assays are based on cooperative assembly of dual proximity probes, and they are highly sensitive and generalizable to essentially any protein with two available antibodies (or aptamers). These assays decouple target binding from the reporting mechanism by exploiting DNA hybridization and/or strand displacement reactions. We propose that our proximity assay format is amenable to two-way communication, rather than the one-way information transfer in typical biosensing systems. The objective of this proposal is to show that surface-based proximity assays can sense hormone levels secreted by endocrine cells, then respond proportionally by releasing agents that modify secretion. Such hormone-responsive electrodes could generate new paradigms in cell culture and allow endocrine tissue to be monitored in more in vivo like, responsive environments. Completion of our objective through the following goals will deliver transformative impacts in biosensing, responsive materials, and synthetic biology: 1) Develop an entirely surface-bound electrochemical proximity assay, 2) Synthesize functional DNA-drug conjugates, and 3) Develop hormone-responsive electrodes for quantitative communication from surfaces, through DNA circuits, and to living endocrine cells. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/96527
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Christopher Easley. Hormone-Responsive Electrodes for Quantitative Discourse with Endocrine Cells. 2013-01-01.
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