| 项目编号: | 1350178
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| 项目名称: | CAREER: Towards Synthetic Biology in Human Stem Cells |
| 作者: | Krishanu Saha
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| 承担单位: | University of Wisconsin-Madison
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| 批准年: | 2013
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| 开始日期: | 2014-06-01
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| 结束日期: | 2019-05-31
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| 资助金额: | USD375000
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| 资助来源: | US-NSF
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| 项目类别: | Continuing grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | stem cell
; human stem cell
; proposal
; cell
; synthetic biology program
; cell behavior
; cell signaling pathway
; novel system
; cellular biology
; new synthetic biology tool
; career award
; mutant cell
; other cell
; synthetic biology
; culture
; synthetic biology tool
|
| 英文摘要: | CBET-1350178
Saha, Krishanu
Human stem cells provide an attractive, patient-matched source for generating many tissues found in the body, but controlling stem cells in culture remains a key challenge. To meet this challenge, this proposal seeks to develop and apply synthetic biology tools in order to dissect and precisely control the complex molecular signaling processes that determine stem cell behavior in culture. Synthetic biology is an emerging biotechnology field that combines elements of engineering, mathematics, chemistry, and biology to synthesize novel systems from characterized biological components. Applying new synthetic biology tools in human stem cells to control cell behavior would enable advanced manufacturing of patient-specific tissues and cells for disease modeling and drug discovery applications. This proposal outlines a novel approach to convert natural signaling components in human stem cells to synthetic, bioorthogonal ones. The approach relies on producing mutations in cells at defined genomic locations. Such an approach allows for the formation of libraries of mutant cells that can be selected for particular functions. The proposal focuses on a fundamental barrier to establishing a robust stem cell tissue engineering and bioprocessing industry ? manipulating cell signaling pathways when stem cells grow in culture and come together with other cells and materials.
The research will be integrated into education by developing and disseminating course modules demonstrating the importance of cross-disciplinary training in stem cell biology, engineering and bioethics for postgenomic approaches to human biomedicine. The proposal to incorporate underrepresented students into the research laboratory and to advance new curriculum will help to develop the next generation of students trained at the stem cell biology-engineering interface.
Due to the interdisciplinary nature of the project, this CAREER award by the Biotechnology, Biochemical, and Biomass Engineering Program of the CBET Division is co-funded by the Systems and Synthetic Biology Program of the Division of Molecular and Cellular Biology. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/96654
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Krishanu Saha. CAREER: Towards Synthetic Biology in Human Stem Cells. 2013-01-01.
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