| 项目编号: | 1351443
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| 项目名称: | CAREER: Revisiting the Central Dogma at the Genome Scale and in Single Cells |
| 作者: | Rong Fan
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| 承担单位: | Yale University
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| 批准年: | 2013
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| 开始日期: | 2014-04-01
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| 结束日期: | 2019-03-31
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| 资助金额: | USD360000
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| 资助来源: | US-NSF
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| 项目类别: | Continuing grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | stem cell fate
; cellular function
; cellular behavior
; same single cell
; single-cell genetic variation
; cellular biology
; immune cell diversity
; career award
; central dogma
; single cell
; stem cell differentiation
; whole genome
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| 英文摘要: | 1351443
Fan, Rong
The ultimate objective of this research project is to develop a microdevice platform that can enable the measurement of a single cell simultaneously for genomic, transcriptomic, proteomic and phenotypic parameters, in other words, at all the levels of the Central Dogma(DNA to RNA to protein to phenotype). Specifically, the PI will develop a microfluidic device for reliably separating cytoplasmic and nuclear contents from the same single cells followed by whole-pool amplification of genomic DNAs and messenger RNAs. The amplified products will be retrieved and analyzed using state-of-the-art genomics technology to link single-cell genetic variation to the expression of affected genes across the whole genome. Single-cell, multiplex protein assay and quantitative analysis of cellular behaviors (phenotype) can be integrated within this microdevice to further correlate genes, transcripts to the levels of proteins and cellular functions. The PI will apply this platform to investigating stem cell differentiation and potentially provide new insights needed for understanding how gene expression is regulated to determine stem cell fate and diversity. Such analysis is critical to fully addresse a range of fundamental biological questions such as embryonic development, cancer and immune cell diversity.
This project also acts as a backbone for the integration of research and educational activities spanning from K12 through graduate training, from Yale University through the local community. Integrating microtechnology into biology represents an urgent requirement of expertise for the future health science researchers and medical practitioners. The synergy of research and education will result in the development of a virtual micro-physiologic system lab for demonstration to not only Yale College students but also to the general public, and increase the participation of minorities and under-represented groups.
Due to the interdisciplinary nature of the project, this CAREER award by the Biotechnology, Biochemical, and Biomass Engineering Program of the CBET Division is co-funded by the Systems and Synthetic Biology Program of the Division of Molecular and Cellular Biology. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/97124
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Rong Fan. CAREER: Revisiting the Central Dogma at the Genome Scale and in Single Cells. 2013-01-01.
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