| 项目编号: | 1419960
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| 项目名称: | EAGER: Developing epigenetic genotyping-by-sequencing in the non-model invasive Japanese knotweed |
| 作者: | Christina Richards
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| 承担单位: | University of South Florida
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| 批准年: | 2013
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| 开始日期: | 2014-03-15
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| 结束日期: | 2017-02-28
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| 资助金额: | USD219995
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Biological Sciences - Environmental Biology
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| 英文关键词: | genotyping-by-sequencing
; technique
; bisulfite sequencing
; novel next generation sequencing technique
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| 英文摘要: | Biologists have long been interested in understanding the mechanistic basis of adaptation. Epigenetic effects (e.g., methylation) can contribute to variation in the traits of organisms even in the complete absence of differences in DNA sequence information. Epigenetic changes in DNA can be induced by environmental stress and lead to a more rapid response to natural selection but there is little information on how epigenetic mechanisms function in natural populations. This project will develop a novel next generation sequencing technique allowing for the measurement of both genetic and epigenetic variation under different environmental conditions in non-model organisms with no reference genome. The technique will combine Genotyping-By-Sequencing and bisulfite sequencing to simultaneously measure DNA sequence and methylation polymorphisms at tens to hundreds of thousands of fragments across the genome. As a proof of principle, the technique will be developed in Fallopia japonica, a highly invasive polyploid plant with a large and complex genome.
The techniques developed in this EAGER will be used in workshops with Hillsborough County Public School teachers and students to allow for effective outreach of the latest in innovative genomics approaches that will be applicable to a wide variety of biological research as well as understanding of social issues like personalized medicine, and genetically modified crops. At the University of South Florida the project will include summer research for undergraduates, and graduate student training. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/97243
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Christina Richards. EAGER: Developing epigenetic genotyping-by-sequencing in the non-model invasive Japanese knotweed. 2013-01-01.
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