| 项目编号: | 1403984
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| 项目名称: | EAGER: Evolving Multipurpose Biological Magnetic Nanoparticles |
| 作者: | Dino Di Carlo
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| 承担单位: | University of California-Los Angeles
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| 批准年: | 2013
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| 开始日期: | 2014-03-01
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| 结束日期: | 2015-02-28
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| 资助金额: | USD150000
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| 资助来源: | US-NSF
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| 项目类别: | Standard Grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | magnetic nanoparticle
; biological system
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| 英文摘要: | 1403984
Di Carlo
Magnetic nanoparticles have been widely used in life science research and medicine. Such particles are used for construction of biosensors, as well as for detection of important immune or cancer cells from physiological samples. Currently, magnetic particles must be synthesized through chemical processes, and proteins or other targeting components are added to particle surfaces through painstaking step-by-step processes. Nature has also developed an approach to biomineralize magnetite to create magnetic nanoparticles. Several species of ?magnetotactic? bacteria evolved to produce chains of magnetic nanoparticles in specialized compartments called magnetosomes that aid in alignment within Earth?s magnetic field. These bacteria may be ideal factories for the next-generation of low cost magnetic particles that are pre-coated with medically useful proteins. We will use microfluidic tools to apply selection pressures for directed evolution of magnetotactic bacteria with a range of magnetosome properties. We will also develop techniques to isolate magnetosomes from the selected cell populations while maintaining the commercially useful protein and lipid envelope surrounding the precipitated magnetite particles. Besides use in creating customized and potentially economical protein-coated nanoparticles for biosensing, imaging, and cell biology research, the genetic changes within our evolved strain will give unique insights into biomineralization, vesicle formation, and self-assembly processes that can be used in a variety of biological systems. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/97297
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Dino Di Carlo. EAGER: Evolving Multipurpose Biological Magnetic Nanoparticles. 2013-01-01.
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