| 项目编号: | 1351531
|
| 项目名称: | CAREER: Artificial Cell Membranes for Ultra-Pure, High Throughput Cellular Isolation |
| 作者: | Brad Berron
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| 承担单位: | University of Kentucky Research Foundation
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| 批准年: | 2013
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| 开始日期: | 2014-02-01
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| 结束日期: | 2019-01-31
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| 资助金额: | USD422512
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| 资助来源: | US-NSF
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| 项目类别: | Continuing grant
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| 国家: | US
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| 语种: | 英语
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| 特色学科分类: | Engineering - Chemical, Bioengineering, Environmental, and Transport Systems
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| 英文关键词: | cell
; high-purity
; career development plan
; asl
; high rate
; cellular protection
; nonspecific cell
; uncoated cell
; high-purity cell sorting
; screencast
; high speed isolation
; non-targeted cell
; cbet-1351531berroncell-based therapy
; rapid cell isolation method
; high-purity isolate
; cellular isolation technique
; cellular isolation
|
| 英文摘要: | CBET-1351531
Berron
Cell-based therapies, where cells are transplanted to naturally restore biological function, represent an exciting future biomedical application. Critically, current techniques for high-purity cell sorting are time-intensive processes which lack the speed to satisfy the emerging widespread need for purified cells in research. The PI recently discovered a rapid cell isolation method in which batch size is virtually unlimited and nonspecific cells are undetected. The developed technology, Antigen Specific Lysis (ASL), selectively coats targeted cells in a temporary protective membrane, then destroys all uncoated cells to yield viable, purified cells. The proposed work will investigate fundamental mechanisms and relationships governing ASL in cellular isolation. This career development plan provides the foundation for a long term research program in the development ASL for the high speed isolation of rare biological products which yields a high-purity isolate from poorly-expressed markers. This research explores fundamental questions of cellular protection, and applies these findings to a cellular isolation technique which has the potential to reduce the presence of non-targeted cells to undetectable levels while sorting at exceptionally high rates.
Bioseparations will play a critical role in the future of the US economy, yet many postsecondary institutions lack sufficient staffing and expertise to offer these courses. A distance learning Bioseparations course will be developed to reach geographically disparate institutions, enabling a robust offering of Bioseparations materials to students several hundreds of miles away. This course will serve as a platform to probe the limits of collaborative learning techniques in distance learning environments. A significant limitation to Bioseparations education is a lack of online learning tools. ASL is built upon a foundation of diverse Bioseparations principles (e.g. lysis, filtration, sedimentation, adsorption), and this career development plan uses ASL as the centerpiece to online Bioseparations screencasts - short educational videos. These videos are popular among students, yet are currently limited in Chemical Engineering to core courses. The developed screencasts will be disseminated in collaboration with the NSF-funded, LearnChemE.com site, where the existing screencasts have had 1.6 million views in the last 12 months. Further, the screencasts will be critiqued and edited by the LearnChemE.com team, ensuring the highest quality educational products. |
| 资源类型: | 项目
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| 标识符: | http://119.78.100.158/handle/2HF3EXSE/97356
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| Appears in Collections: | 影响、适应和脆弱性
气候减缓与适应
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| Recommended Citation: | |
Brad Berron. CAREER: Artificial Cell Membranes for Ultra-Pure, High Throughput Cellular Isolation. 2013-01-01.
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