alpha-Tthalassemia mental retardation X-linked (ATRX) is a chromatin remodeler frequently mutated in many cancers. Despite the binding pattern of ATRX in heterochromatin, ATRX-mediated epigenomic changes in cancer cells have not been profiled, especially for the heterochromatin regions. Here, we profiled genome-wide maps of chromatin accessibility in ATRX-intact and ATRX-null human cancer cells. We found extensive changes in chromatin accessibility in both repetitive DNA regions and non-repetitive regulatory regions following ATRX loss. These changes are highly correlated with changes in transcription, which lead to alterations in cancer-related signalling pathways, such as upregulation of the TGF-beta pathway and downregulation of the cadherin family of proteins. These findings indicate that ATRX deficiency induces epigenomic changes and promotes tumorigenesis through both genome instability and shifts in transcription.
1.Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, State Key Lab Med Mol Biol, Beijing 100005, Peoples R China 2.Chinese Acad Med Sci, Canc Hosp, Peking Union Med Coll, Natl Canc Ctr, Beijing, Peoples R China
Recommended Citation:
Liang, Junbo,Liu, Hongchao,Li, Guangyu,et al. Global changes in chromatin accessibility and transcription following ATRX inactivation in human cancer cells[J]. FEBS LETTERS,2019-01-01