Hepatocellular carcinoma (HCC) is one of the most prevalent malignant diseases worldwide and Hepatic B virus infection is the major risk factor in east-Asia and Sub-Saharan Africa. Hepatitis B virus-encoded X protein (HBX) has been widely reported to play important and extensive roles in the pathogenesis of HCC. And some microRNAs (miRNAs) are getting more attentions in carcinogenesis of HCC. However, the global changes of miRNA expression in hepatocellular induced by HBX protein have been less studied. Here, we transfected the HBx gene into L02 and HepG2 cells using lentivirus, and investigated the influence of HBX protein on the miRNA expression by miRNA microarray assay. We found 19 significantly different expressed miRNAs and predicted 304 potential target genes regulated by these miRNAs. Using quantitative RT-PCR, we identified that the different expression of miRNA-21, miRNA-211 and miRNA-125b in HepG2-HBX cell and HCC tissues was consistent with the results of miRNA microarray and the expression level of these miRNAs target gene also changed. In conclusion, we have comprehensively analyzed the global influence of HBX protein on hepatocyte miRNA expression and found more miRNA targets induced by HBX protein.