globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1902010116
论文题名:
Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes
作者: Do T.H.T.; Choi H.; Palmgren M.; Martinoia E.; Hwang J.-U.; Lee Y.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:25
起始页码: 12540
结束页码: 12549
语种: 英语
英文关键词: ABC transporters ; AtABCG28 ; Pollen tube growth ; Polyamine ; ROS
Scopus关键词: ABC transporter ; polyamine ; reactive oxygen metabolite ; Arabidopsis thaliana ; Article ; AtABCG28 gene ; cell membrane ; cell migration ; cell wall ; controlled study ; gene ; gene expression ; gene function ; gene knockdown ; gene location ; germination ; in vitro study ; nonhuman ; pollen tube ; priority journal ; secretory vesicle
英文摘要: Tip-focused accumulation of reactive oxygen species (ROS) is tightly associated with pollen tube growth and is thus critical for fertilization. However, it is unclear how tip-growing cells establish such specific ROS localization. Polyamines have been proposed to function in tip growth as precursors of the ROS, hydrogen peroxide. The ABC transporter AtABCG28 may regulate ROS status, as it contains multiple cysteine residues, a characteristic of proteins involved in ROS homeostasis. In this study, we found that AtABCG28 was specifically expressed in the mature pollen grains and pollen tubes. AtABCG28 was localized to secretory vesicles inside the pollen tube that moved toward and fused with the plasma membrane of the pollen tube tip. Knocking out AtABCG28 resulted in defective pollen tube growth, failure to localize polyamine and ROS to the growing pollen tube tip, and complete male sterility, whereas ectopic expression of this gene in root hair could recover ROS accumulation at the tip and improved the growth under high-pH conditions, which normally prevent ROS accumulation and tip growth. Together, these data suggest that AtABCG28 is critical for localizing polyamine and ROS at the growing tip. In addition, this function of AtABCG28 is likely to protect the pollen tube from the cytotoxicity of polyamine and contribute to the delivery of polyamine to the growing tip for incorporation into the expanding cell wall. © 2019 National Academy of Sciences. All rights reserved.
Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163551
Appears in Collections:气候变化与战略

Files in This Item:

There are no files associated with this item.


作者单位: Do, T.H.T., Division of Integrative Bioscience and Biotechnology, POSTECH, Pohang, 37673, South Korea; Choi, H., Division of Integrative Bioscience and Biotechnology, POSTECH, Pohang, 37673, South Korea; Palmgren, M., Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, DK-1871, Denmark; Martinoia, E., Division of Integrative Bioscience and Biotechnology, POSTECH, Pohang, 37673, South Korea, Department of Plant and Microbial Biology, University of Zurich, Zurich, 8008, Switzerland; Hwang, J.-U., Division of Integrative Bioscience and Biotechnology, POSTECH, Pohang, 37673, South Korea; Lee, Y., Division of Integrative Bioscience and Biotechnology, POSTECH, Pohang, 37673, South Korea

Recommended Citation:
Do T.H.T.,Choi H.,Palmgren M.,et al. Arabidopsis ABCG28 is required for the apical accumulation of reactive oxygen species in growing pollen tubes[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(25)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Do T.H.T.]'s Articles
[Choi H.]'s Articles
[Palmgren M.]'s Articles
百度学术
Similar articles in Baidu Scholar
[Do T.H.T.]'s Articles
[Choi H.]'s Articles
[Palmgren M.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Do T.H.T.]‘s Articles
[Choi H.]‘s Articles
[Palmgren M.]‘s Articles
Related Copyright Policies
Null
收藏/分享
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.