globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1721604115
论文题名:
Converting Escherichia coli into an archaebacterium with a hybrid heterochiral membrane
作者: Caforio A.; Siliakus M.F.; Exterkate M.; Jain S.; Jumde V.R.; Andringa R.L.H.; Kengen S.W.M.; Minnaard A.J.; Driessen A.J.M.; van der Oost J.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:14
起始页码: 3704
结束页码: 3709
语种: 英语
英文关键词: Archaea ; Bacteria ; Ether lipids ; Hybrid membranes ; Lipid biosynthesis
Scopus关键词: ether phospholipid ; glycerophosphate ; isoprenoid ; ether derivative ; membrane lipid ; phospholipid ; archaeon ; Article ; bacterial cell ; bacterial membrane ; bacterial strain ; cell differentiation ; cell engineering ; cell growth ; chirality ; controlled study ; Escherichia coli ; lipid analysis ; lipid level ; lipogenesis ; nonhuman ; phenotype ; priority journal ; stereochemistry ; archaeon ; cell membrane ; chemistry ; Escherichia coli ; evolution ; metabolism ; Archaea ; Biological Evolution ; Cell Membrane ; Escherichia coli ; Ethers ; Membrane Lipids ; Phospholipids
英文摘要: One of the main differences between bacteria and archaea concerns their membrane composition. Whereas bacterial membranes are made up of glycerol-3-phosphate ester lipids, archaeal membranes are composed of glycerol-1-phosphate ether lipids. Here, we report the construction of a stable hybrid heterochiral membrane through lipid engineering of the bacterium Escherichia coli. By boosting isoprenoid biosynthesis and heterologous expression of archaeal ether lipid biosynthesis genes, we obtained a viable E. coli strain of which the membranes contain archaeal lipids with the expected stereochemistry. It has been found that the archaeal lipid biosynthesis enzymes are relatively promiscuous with respect to their glycerol phosphate backbone and that E. coli has the unexpected potential to generate glycerol-1-phosphate. The unprecedented level of 20–30% archaeal lipids in a bacterial cell has allowed for analyzing the effect on the mixed-membrane cell’s phenotype. Interestingly, growth rates are unchanged, whereas the robustness of cells with a hybrid heterochiral membrane appeared slightly increased. The implications of these findings for evolutionary scenarios are discussed. © 2018 National Academy of Sciences. All Rights Reserved.
Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163728
Appears in Collections:气候变化与战略

Files in This Item:

There are no files associated with this item.


作者单位: Caforio, A., Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, 9747 AG, Netherlands, Zernike Institute for Advanced Materials, University of Groningen, Groningen, 9747 AG, Netherlands; Siliakus, M.F., Laboratory of Microbiology, Wageningen University & Research, Wageningen, 6708 WE, Netherlands; Exterkate, M., Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, 9747 AG, Netherlands, Zernike Institute for Advanced Materials, University of Groningen, Groningen, 9747 AG, Netherlands; Jain, S., Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, 9747 AG, Netherlands, Zernike Institute for Advanced Materials, University of Groningen, Groningen, 9747 AG, Netherlands, Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, Boston, MA 02118, United States; Jumde, V.R., Stratingh Institute for Chemistry, University of Groningen, Groningen, 9747 AG, Netherlands; Andringa, R.L.H., Stratingh Institute for Chemistry, University of Groningen, Groningen, 9747 AG, Netherlands; Kengen, S.W.M., Laboratory of Microbiology, Wageningen University & Research, Wageningen, 6708 WE, Netherlands; Minnaard, A.J., Stratingh Institute for Chemistry, University of Groningen, Groningen, 9747 AG, Netherlands; Driessen, A.J.M., Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, 9747 AG, Netherlands, Zernike Institute for Advanced Materials, University of Groningen, Groningen, 9747 AG, Netherlands; van der Oost, J., Laboratory of Microbiology, Wageningen University & Research, Wageningen, 6708 WE, Netherlands

Recommended Citation:
Caforio A.,Siliakus M.F.,Exterkate M.,et al. Converting Escherichia coli into an archaebacterium with a hybrid heterochiral membrane[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(14)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Caforio A.]'s Articles
[Siliakus M.F.]'s Articles
[Exterkate M.]'s Articles
百度学术
Similar articles in Baidu Scholar
[Caforio A.]'s Articles
[Siliakus M.F.]'s Articles
[Exterkate M.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Caforio A.]‘s Articles
[Siliakus M.F.]‘s Articles
[Exterkate M.]‘s Articles
Related Copyright Policies
Null
收藏/分享
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.