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globalchange > 过去全球变化的重建

DOI:	10.1371/journal.pone.0110753
论文题名:	Post Mortem DNA Degradation of Human Tissue Experimentally Mummified in Salt
作者:	Natallia Shved; Cordula Haas; Christina Papageorgopoulou; Guelfirde Akguel; Katja Paulsen; Abigail Bouwman; Christina Warinner; Frank Rühli
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-10-22
卷:	9, 期:10
语种:	英语
英文关键词:	Ancient DNA ; DNA fragmentation ; DNA damage ; Polymerase chain reaction ; Sequence alignment ; Mitochondria ; Mitochondrial DNA ; DNA extraction
英文摘要:	Mummified human tissues are of great interest in forensics and biomolecular archaeology. The aim of this study was to analyse post mortem DNA alterations in soft tissues in order to improve our knowledge of the patterns of DNA degradation that occur during salt mummification. In this study, the lower limb of a female human donor was amputated within 24 h post mortem and mummified using a process designed to simulate the salt dehydration phase of natural or artificial mummification. Skin and skeletal muscle were sampled at multiple time points over a period of 322 days and subjected to genetic analysis. Patterns of genomic fragmentation, miscoding lesions, and overall DNA degradation in both nuclear and mitochondrial DNA was assessed by different methods: gel electrophoresis, multiplex comparative autosomal STR length amplification, cloning and sequence analysis, and PCR amplification of different fragment sizes using a damage sensitive recombinant polymerase. The study outcome reveals a very good level of DNA preservation in salt mummified tissues over the course of the experiment, with an overall slower rate of DNA fragmentation in skin compared to muscle.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0110753&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/19025
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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journal.pone.0110753.PDF（1550KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Institute of Evolutionary Medicine, University of Zurich, Zurich, Switzerland;Department of Forensic Genetics, Institute of Legal Medicine, University of Zürich, Zürich, Switzerland;Institute of Evolutionary Medicine, University of Zurich, Zurich, Switzerland;Institute of Evolutionary Medicine, University of Zurich, Zurich, Switzerland;Division of Cell- and Neurobiology, Institute of Anatomy, University of Zurich, Zurich, Switzerland;Institute of Evolutionary Medicine, University of Zurich, Zurich, Switzerland;Institute of Evolutionary Medicine, University of Zurich, Zurich, Switzerland;Institute of Evolutionary Medicine, University of Zurich, Zurich, Switzerland

Recommended Citation:

Natallia Shved,Cordula Haas,Christina Papageorgopoulou,et al. Post Mortem DNA Degradation of Human Tissue Experimentally Mummified in Salt[J]. PLOS ONE,2014-01-01,9(10)

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